Mice lacking Sμ tandem repeats maintain RNA polymerase patterns but exhibit histone modification pattern shifts linked to class switch site locations.

Molecular Immunology
Barbara B BalterErik Selsing

Abstract

Antibody switching involves class switch recombination (CSR) events between switch (S) regions located upstream of heavy chain constant (C) genes. Mechanisms targeting CSR to S-regions are not clear. Deletion of Sμ tandem repeat (SμTR) sequences causes CSR to shift into downstream regions that do not undergo CSR in WT B-cells, including the Cμ-region. We now find that, in SμTR(-/-) B cells, Sμ chromatin histone modification patterns also shift downstream relative to WT and coincide with SμTR(-/-) CSR locations. Our results suggest that histone H3 acetylation and methylation are involved in accessibility of switch regions and that these modifications are not dependent on the underlying sequence, but may be controlled by the location of upstream promoter or regulatory elements. Our studies also show RNA polymerase II (RNAPII) loading increases in the Eμ/Iμ region in stimulated B cells; these increases are independent of SμTR sequences. Longer Sμ deletions have been reported to eliminate increases in RNAPII density, therefore we suggest that sequences between Iμ and Sμ (possibly the Iμ splicing region as well as G-tracts that are involved in stable RNA:DNA complex formation during transcription) might control the RNAPII density in...Continue Reading

References

Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J StavnezerE Severinson
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Cristina RadaCésar Milstein
Jul 5, 2002·Nature·Svend K Petersen-MahrtMichael S Neuberger
Apr 15, 2003·Nature·Jayanta ChaudhuriFrederick W Alt
Sep 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Katrina B MorsheadMarjorie A Oettinger
Apr 21, 2004·Nature Structural & Molecular Biology·Qin YuNathaniel R Landau
Oct 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ziqiang LiMatthew D Scharff
Oct 19, 2004·Genes & Development·Robert J SimsDanny Reinberg
Aug 17, 2005·The Journal of Experimental Medicine·Carol E SchraderJanet Stavnezer
Jan 19, 2006·The Journal of Experimental Medicine·Lili WangAmy L Kenter
Feb 27, 2007·Cell·Tony Kouzarides
Jun 15, 2007·Molecular and Cellular Biology·Feng-Ting HuangMichael R Lieber
Mar 29, 2008·Annual Review of Immunology·Janet StavnezerCarol E Schrader
Oct 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Muslima ChowdhuryDavid J Fear
Mar 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Fei Li KuangMatthew D Scharff
May 13, 2009·The Journal of Experimental Medicine·Deepa RajagopalPatricia J Gearhart
Dec 9, 2010·Proceedings of the National Academy of Sciences of the United States of America·Andre StanlieNasim A Begum
Feb 2, 2011·Molecular and Cellular Biology·Kristopher R SchwabGregory R Dressler

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Citations

May 15, 2013·Molecular Cell·Jeremy A Daniel, André Nussenzweig
Nov 19, 2013·Journal of Biomedical Materials Research. Part a·Xuezi SangFashui Hong
Sep 12, 2019·Critical Reviews in Biochemistry and Molecular Biology·Kefei Yu, Michael R Lieber

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