Mice lacking the 65 kDa isoform of glutamic acid decarboxylase (GAD65) maintain normal levels of GAD67 and GABA in their brains but are susceptible to seizures

Biochemical and Biophysical Research Communications
H AsadaK Obata

Abstract

The gene encoding of the 65 kDa isoform of the gamma-aminobutyric acid (GABA)-synthesizing enzyme, glutamic acid decarboxylase (GAD), GAD65, was targeted in mice by homologous recombination. Viable GAD65 -/- mice were obtained with the expected mendelian frequency and displayed no gross morphological defects. Despite the complete loss of GAD65 mRNA and protein in a homozygous mutant, there was no difference in GABA content in the brains of GAD65 +/+, +/-, and -/- mice. As for the other 67 kDa isoform (GAD67), the levels of mRNA and protein were largely unchanged by the GAD65 mutation. General behavior, including locomotor activity and performance in the Morris water maze task, appeared normal, but seizures were more easily induced by picrotoxin and pentylenetetrazol: the latencies to seizures induced by picrotoxin were shorter and the dose of pentylenetetrazol required for induction of seizures was lower.

Citations

Oct 21, 2009·Molecular Biology Reports·Graham RichardsonJianyong Li
Jul 7, 2011·Nature Communications·Inseon SongAlexey Semyanov
Apr 2, 2005·Nature Reviews. Neuroscience·David A LewisDavid W Volk
Mar 27, 2007·Nature Structural & Molecular Biology·Gustavo FenaltiJames C Whisstock
Oct 6, 2012·The Journal of Biological Chemistry·Pingyang LiuJianyong Li
Aug 27, 2010·Cerebral Cortex·Carlos Sánchez-Huertas, Beatriz Rico
Jan 7, 2010·Neuroreport·Lichao ChenRitchie E Brown
Sep 19, 2006·Molecular and Cellular Biology·Alison O'MahonyWarner C Greene
Apr 2, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yi HeWeihong Pan
Apr 12, 2013·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Kunihiko Obata
Jun 17, 2006·Journal of Neurosurgery·Shenandoah RobinsonMark L Cohen
Sep 10, 2011·Neural Plasticity·Guillermo Gonzalez-BurgosDavid A Lewis
Aug 10, 2011·Neural Plasticity·Stephen W Briggs, Aristea S Galanopoulou
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·H AsadaK Obata
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·N TianR Nicoll
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·S F KashS Baekkeskov
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Nobuko MatagaTakao K Hensch
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·S F KashS Baekkeskov
Mar 26, 2014·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Maren D LangeHans-Christian Pape
Oct 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Eun-Jin YangTakao K Hensch
Dec 7, 2006·Developmental Neuroscience·Gabriela J MartinsElizabeth M Powell
Sep 2, 2010·The Journal of Cell Biology·Jamil KanaaniSteinunn Baekkeskov
Nov 7, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·Suren B BandaraSusan L Schantz
May 10, 2012·Behavioural Brain Research·Monica M BoltonJefferson W Kinney
Dec 14, 2011·Trends in Neurosciences·David A LewisDavid W Volk
Jan 12, 2010·Neuroscience and Biobehavioral Reviews·Suat Ying Tan CherlynKang Sim
Aug 2, 2008·Journal of Chemical Neuroanatomy·Ya-Yun WangYun-Qing Li
Jan 19, 2016·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·M I CorderoC Sandi
Sep 24, 2015·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Jinwook KimChangjong Moon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.