Mice with targeted gene disruptions or gene insertions for diabetes research: problems, pitfalls, and potential solutions

Diabetologia
E H Leiter

Abstract

The mouse has been a favoured organism for molecular manipulation in studies seeking to establish the genetic bases and pathophysiologic mechanisms underlying both Type I (insulin-dependent) and Type II (non-insulin-dependent) diabetes mellitus. Gene targeting and transgenesis are the two powerful molecular technologies used in these endeavours. Interpretation of results generated from such studies, either entailing an altered phenotype or the absence of a phenotypic change, is not always simple. This review focuses on certain complications inherent in the methodologies, and outlines steps that can be taken to distinguish effects of the genetic manipulation from unexpected contributions from the genetic background.

Citations

Oct 25, 2002·Diabetes/metabolism Research and Reviews
Nov 2, 2005·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Jing ChenEdward H Leiter
Apr 6, 2006·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Nicola J ArmstrongTerence P Speed
Mar 30, 2005·Current Diabetes Reports·Edward H Leiter
Nov 5, 2002·Current Opinion in Immunology·Grant Morahan, Laurence Morel
Jun 6, 2003·Current Opinion in Genetics & Development·Roger D Cox, Steve D M Brown
Jun 21, 2007·Nature Immunology·William M RidgwayLinda S Wicker
Jun 29, 2006·ILAR Journal·Nicole Neubauer, Rohit N Kulkarni
Oct 9, 2007·Diabetes, Obesity & Metabolism·E H LeiterA Chervonsky
Nov 5, 2010·Diabetes, Obesity & Metabolism·J Pi, S Collins
May 1, 2007·Annual Review of Nutrition·Xin Gen LeiJames P McClung
May 30, 2013·PloS One·Siiri E IismaaRobert M Graham
Jun 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·James P McClungXin Gen Lei
Apr 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Brad J MarshKathryn E Howell
Dec 2, 2004·Canadian Journal of Physiology and Pharmacology·David L Severson
Oct 6, 2009·Seminars in Immunopathology·Bettina SchreinerBurkhard Becher
Jun 25, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jorge CastroMriganka Sur
Dec 3, 2003·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·A L DiamentC H Warden
Dec 18, 2003·Annals of the New York Academy of Sciences·Decio L EizirikAlessandra K Cardozo
Nov 3, 2006·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Kirsten DahlAxel K Hansen
Mar 3, 2007·Annals of the New York Academy of Sciences·Leonard D ShultzDale L Greiner
Dec 17, 2014·Diabetologia·Bernard ThorensJorge Ferrer
Dec 25, 2003·Diabetes·William H SchottEdward H Leiter
Sep 9, 2015·PLoS Genetics·Oliver KluthAnnette Schürmann
Nov 3, 2004·Journal of Lipid Research·Sandrine DubracSandra K Erickson
Jan 18, 2008·Journal of Lipid Research·Sandra K Erickson
Jul 20, 2013·Journal of Nursing Management·Carol A Wong, Lisa M Giallonardo
Oct 24, 2014·American Journal of Speech-language Pathology·Susan NittrouerJoanna H Lowenstein
May 17, 2008·Biochemical Society Transactions·Conny GysemansChantal Mathieu
Dec 14, 2019·Journal of Speech, Language, and Hearing Research : JSLHR·Susan NittrouerJoseph Antonelli
Oct 7, 2015·G3 : Genes - Genomes - Genetics·Colleen M ElsoThomas C Brodnicki

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