Micellar and sub-micellar liquid chromatography of terephthalic acid contaminants using a C18 column coated with Tween 20

Analytica Chimica Acta
Abd Al-Karim F Ali, Neil D Danielson

Abstract

The tremendous amounts of terephthalic acid (TPA) produced globally require consistent monitoring of its contaminants during the different stages of production for quality control purposes. In this paper, a simple, robust and green liquid chromatography method has been developed using an isocratic 100% aqueous mobile phase at pH 2 (dilute sulfuric acid) to separate TPA contaminants (mono-, di-, and tri-carboxylic aromatic acids) on a C18 stationary phase coated with Tween 20 (polyoxyethylene(20)sorbitan monolaurate). After optimization of all chromatographic conditions, near baseline separation of the nine carboxylic acids under investigation was achieved with a 2.5 mL/min flow rate on a 5 micron C18 silica column (100 x 4.6 mm) in under 20 min. The modified stationary phase showed an excellent capability to separate structural isomers in a reasonable time, markedly better that the bare C18 stationary phase. Plots of ln retention factor versus 1/temperature showed the expected linear relationship for the di- and tri-carboxylic aromatic acids (single retention mechanism likely) but a quadratic fit for the mono-carboxylic aromatic acids (dual retention mechanism likely). Due to the stability of the surfactant modified stationary ...Continue Reading

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