Micellar delivery of dasatinib for the inhibition of pathologic cellular processes of the retinal pigment epithelium

Colloids and Surfaces. B, Biointerfaces
Qingqing LiThomas W Y Lee

Abstract

The objective of this study was to fabricate dasatinib-loaded nanoparticles and evaluate their efficacy in inhibiting cellular processes of the retinal pigment epithelium (RPE) related to proliferative vitreoretinopathy (PVR), for which there are no approved pharmacological approaches. We successfully encapsulated dasatinib, a poorly soluble multi-targeted tyrosine kinase inhibitor which has great potential for the treatment of PVR, into nanoparticles prepared from micellation of PEG-b-PCL. The size of the nanomicelles was approximately 55nm with a narrow distribution. They increased the solubility of dasatinib by 475× and provided a sustained drug release. ARPE-19, an immortal RPE cell line, was used to assess the in vitro efficacy of micellar dasatinib because the RPE is believed to play a key role in the pathogenesis of PVR. Three cell-based assays, namely, proliferation, adhesion and migration, which represent three important PVR-related cellular changes of the RPE, were conducted and the cytotoxicity of micelles was also evaluated. Both blank and dasatinib-loaded micelles were non-cytotoxic towards ARPE-19 cells. Micellar dasatinib significantly inhibited cell proliferation, adhesion and migration compared to the free drug...Continue Reading

References

Sep 27, 2001·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·D Maurice
Jan 5, 2002·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·T W Lee, J R Robinson
Mar 22, 2002·Progress in Retinal and Eye Research·J Carlos PastorFrancisco Martín
Apr 23, 2004·Ophthalmic Research·Christoph W SpraulGabriele E Lang
Nov 22, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·M Laird ForrestGlen S Kwon
Dec 31, 2005·Investigative Ophthalmology & Visual Science·Kirsten H EiblUlrich Welge-Lussen
Apr 13, 2006·Nano Letters·B Devika ChithraniWarren C W Chan
Feb 24, 2009·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Thomas Wai-Yip Lee, Joseph R Robinson
May 5, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ho-Chul ShinGlen S Kwon
Nov 7, 2009·Journal of Pharmaceutical Sciences·Yahya E ChoonaraLisa C du Toit
Dec 21, 2010·Drug Discovery Today·Thilini Rasika ThrimawithanaRaid Ghassan Alany
Apr 7, 2011·Chemical Communications : Chem Comm·Lifeng CaiKeliang Liu
Nov 1, 2011·The American Journal of Pathology·Steven PennockAndrius Kazlauskas
Apr 18, 2012·Macromolecular Bioscience·Shengyan LiuFrank X Gu
Jan 15, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Wai-Leung Langston Suen, Ying Chau
Jan 24, 2013·Investigative Ophthalmology & Visual Science·Kazuhiko UmazumeShigeo Tamiya
Aug 14, 2013·Colloids and Surfaces. B, Biointerfaces·Lu XuXingyi Li
Nov 14, 2013·Expert Opinion on Drug Delivery·Stephen G SchwartzMichael W Stewart
Jan 15, 2014·Progress in Retinal and Eye Research·Steven PennockAndrius Kazlauskas
Jun 17, 2014·Expert Opinion on Drug Delivery·Sachin S ThakurHarendra S Parekh
Oct 14, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·M Naveed YasinIlva D Rupenthal

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Citations

Oct 27, 2017·Pharmaceutical Development and Technology·Dinghu ZhangQin Liu
Sep 21, 2018·Journal of Nanobiotechnology·Jayanta Kumar PatraHan-Seung Shin
Sep 11, 2019·Journal of Microencapsulation·Aashu GuptaManju Misra
Jun 6, 2020·Molecular Pharmaceutics·Qingqing LiShing Fung Chow
Jan 24, 2021·International Journal of Pharmaceutics·Qingqing LiWai Yip Thomas Lee
Apr 21, 2019·Nanomedicine : Nanotechnology, Biology, and Medicine·Qing YaoLin Zhu
Sep 28, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Kritika Nayak, Manju Misra
May 26, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Philip GrossenJörg Huwyler
Jul 3, 2021·International Journal of Molecular Sciences·Eleonora RussoChiara Brullo
Feb 11, 2020·Current Stem Cell Research & Therapy·Nanxin LiuGang Fu

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