Micellar electrokinetic chromatography method for measuring amino acid secretions from islets of Langerhans

Electrophoresis
Xue WangMichael G Roper

Abstract

Islets of Langerhans are responsible for maintaining glucose homeostasis through regulated secretion of hormones and other factors. It is hypothesized that amino acids secreted from islets play a critical role in cell functionality and viability. For example, glutamate and gamma-aminobutyric acid have been proposed to work as paracrine signaling molecules within islets to coordinate the release of hormone secretion; other amino acids, such as glutamine, leucine, alanine, and arginine, have been shown to stimulate or potentiate glucose-stimulated insulin secretion. To characterize the potential roles that these small molecules may play in islet physiology, derivatization of amino acids in high-salt buffers commonly used in islet experiments with naphthalene-2,3-dicarboxaldehyde and MEKC separation conditions were optimized. The optimized conditions used d-norvaline as the internal standard and allowed quantification of 14 amino acids with LODs ranging from 0.2 to 7 nM. The RSDs of the migration times were 0.04-0.54% and the RSDs of the peak areas were 0.2-5.8% for the various amino acids. The effects of glucose and 2,4-dinitrophenol on amino acid secretions from islets were tested and a suppressive effect of glucose on gamma-ami...Continue Reading

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Jun 24, 2014·Analytical Methods : Advancing Methods and Applications·Xue Wang, Michael G Roper

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Citations

Jun 17, 2016·Annual Review of Analytical Chemistry·Adrian M SchrellMichael G Roper
Aug 15, 2017·Electrophoresis·Véréna PoinsotFrançois Couderc
Apr 10, 2019·Critical Reviews in Analytical Chemistry·Raquel Pérez-MíguezMaría Luisa Marina
May 11, 2019·Analytical Methods : Advancing Methods and Applications·Kimberly EvansMichael G Roper

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