Micellar gradients in size-exclusion simulated moving bed chromatography

Journal of Chromatography. a
D A HornemanL A M van der Wielen

Abstract

The selectivity of size-exclusion chromatography (SEC) can be modified by adding non-ionic micelles to the mobile phase. Surfactant-aided size-exclusion chromatography (SASEC) can therefore very well be performed in a gradient mode on an SMB, as is reported in this paper. A method has been developed for correctly positioning a micellar gradient over an SMB. The method is applied for size-exclusion chromatography with the non-ionic surfactant C12E23 as gradient forming solute, and demonstrated by applying it to a relevant chromatographic protein separation problem.

References

Aug 15, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·J C Bosma, J A Wesselingh
Jun 13, 2001·Journal of Colloid and Interface Science·Matthew J. LazzaraWilliam M. Deen
Jul 19, 2001·Journal of Chromatography. a·O Di GiovanniR M Nicoud
Feb 8, 2002·Journal of Chromatography. a·Stefanie AbelMassimo Morbidelli
Feb 8, 2002·Journal of Chromatography. a·Dorota Antos, Andreas Seidel-Morgenstern
Jun 18, 2002·Journal of Chromatography. a·Joukje HouwingLuuk A M van der Wielen
Feb 7, 2004·Journal of Chromatography. a·Stefanie AbelMassimo Morbidelli
Jun 5, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·D A HornemanL A M van der Wielen
Sep 29, 2005·Journal of Chromatography. a·Andreas GeisserBernd Stahl

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Citations

Jun 5, 2007·Journal of Chromatography. a·D A HornemanL A M van der Wielen
Jul 16, 2013·Trends in Biotechnology·Alois Jungbauer

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