Micellar Stability in Biological Media Dictates Internalization in Living Cells

Journal of the American Chemical Society
Natalia Feiner-GraciaLorenzo Albertazzi

Abstract

The dynamic nature of polymeric assemblies makes their stability in biological media a crucial parameter for their potential use as drug delivery systems in vivo. Therefore, it is essential to study and understand the behavior of self-assembled nanocarriers under conditions that will be encountered in vivo such as extreme dilutions and interactions with blood proteins and cells. Herein, using a combination of fluorescence spectroscopy and microscopy, we studied four amphiphilic PEG-dendron hybrids and their self-assembled micelles in order to determine their structure-stability relations. The high molecular precision of the dendritic block enabled us to systematically tune the hydrophobicity and stability of the assembled micelles. Using micelles that change their fluorescent properties upon disassembly, we observed that serum proteins bind to and interact with the polymeric amphiphiles in both their assembled and monomeric states. These interactions strongly affected the stability and enzymatic degradation of the micelles. Finally, using spectrally resolved confocal imaging, we determined the relations between the stability of the polymeric assemblies in biological media and their cell entry. Our results highlight the importan...Continue Reading

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Citations

Aug 10, 2018·Chemical Society Reviews·Abraham J P TeunissenWillem J M Mulder
Feb 20, 2020·Proceedings of the National Academy of Sciences of the United States of America·Hao SuHonggang Cui
Mar 21, 2020·Journal of the American Chemical Society·Shixian LvJianjun Cheng
Dec 28, 2018·ACS Applied Materials & Interfaces·Nathalia Rodrigues de AlmeidaMartin Conda Sheridan
Jan 26, 2021·ACS Applied Bio Materials·Natalia Feiner-GraciaLorenzo Albertazzi
Jul 10, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Kanaparedu P C SekharRati Ranjan Nayak
Sep 7, 2021·Chemical Communications : Chem Comm·Hongxu LiuS Thayumanavan

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