Micellization-induced amplified fluorescence response for highly sensitive detection of heparin in serum.

Scientific Reports
Yeon Jin JangSeoung Ho Lee

Abstract

Fluorescence-based assays should be feasible in aqueous media for effectively detecting the biological factors. However, numerous sensors have limited signal transductions and low fluorescence quantum yields due to the ingerently reduced excited state energy of fluorophores in aqueous solution, which reduces their sensitivity. This necessitates a smart sensing approach with an amplified fluorescence response for analytes in aqueous solution. Herein, a new building block which self-assembles in aqueous media, giving a micellar sturcuture with the hydrophobic π-extended conjugated system at the core and hydrophilic groups at the periphery, was devised for the first time. We demonstrated that the aggregated fluorophores in a micelle induce amplified fluorescence quenching, in which the excited electron efficiently migrates through π-extended conjugated system in a micelle, as in a polymeric system. Such feature differentiates this sensing approach from the numerous fluorescence-based tools previously developed for sensitive detection. This new system exhibited highly sensitive signal transduction for specific analytes even under actual bioanalytical conditions.

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Citations

Apr 23, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jaskiran KaurKuppusamy Gowthamarajan
May 28, 2021·International Journal of Analytical Chemistry·Binjie LiMingjia Yu

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Methods Mentioned

BETA
AFM
dynamic light scattering
transmission
scanning
Atomic
biosensor
column chromatography
X-ray

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