PMID: 7519595Aug 1, 1994Paper

micF antisense RNA has a major role in osmoregulation of OmpF in Escherichia coli

Journal of Bacteriology
N RamaniM Freundlich

Abstract

micF RNA, produced from a multicopy plasmid, was originally shown to be a major factor in negative osmoregulation of the OmpF outer membrane protein in Escherichia coli. However, subsequent experiments with a micF deletion strain suggested that chromosomal micF RNA was not a key component in this process. We report here that micF RNA is essential for the reduction in OmpF levels in cells grown in media of low-to-intermediate levels of osmolarity. Under these conditions, the amount of OmpF was reduced up to 60% in the parent strain while OmpF levels were not altered in the micF deletion mutant. In medium of higher osmolarity, OmpF synthesis was strongly inhibited in both strains. RNA measurements showed that micF RNA levels rose rapidly in cells grown in low-to-intermediate levels of osmolarity concomitant with the reduction in OmpF protein, while ompF mRNA decreased strongly only during high-osmolarity conditions. Taken together, these results strongly suggest that the negative osmoregulation of OmpF at low-to-intermediate osmolarity levels requires micF RNA and that this is masked at higher osmolarity by the known strong inhibition of OmpF transcription by OmpR. Results consistent with this model were also obtained by using pr...Continue Reading

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Citations

Apr 4, 2014·Microbiology·Charlotte MichauxJean-Christophe Giard
Dec 20, 2005·Molecular Microbiology·Maude Guillier, Susan Gottesman
May 27, 2014·Applied and Environmental Microbiology·A MetrisJ Baranyi
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·S GottesmanD J FitzGerald
Feb 1, 2011·Journal of Bacteriology·Asaf Peer, Hanah Margalit
Dec 4, 2019·Nature Reviews. Microbiology·Julia VergalliJean-Marie Pagès
Oct 24, 2009·Biochemistry·Steven D BrownM G Finn
Oct 17, 2001·Journal of Molecular Biology·N Delihas, S Forst
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn

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