Michael Addition Based Chemodosimeter for Serum Creatinine Detection Using ( E)-3-(Pyren-2-yl)-1-(3,4,5-trimethoxyphenyl)prop-2-en-1-one Chalcone

ACS Sensors
Ellairaja SundaramVairathevar Sivasamy Vasantha

Abstract

First, a simple and highly emissive fluorescent chalcone ( E)-3-(pyren-2-yl)-1-(3,4,5-trimethoxyphenyl)prop-2-en-1-one (PTP) was synthesized via simple shaking along with an excellent quantum yield of 0.85, and proved as a stable, highly sensitive, and selective biosensor for creatinine. Owing to its unique photophysical interaction with creatinine through Michael adduct formation, PTP was utilized as a Chemodosimeter for the selective recognition of creatinine in blood serum. Under optimized conditions, a broad range of creatinine detection was achieved  from 0.00000113 mg/dL to 15.8 mg/dL along with an excellent limit of detection of 0.00000065 mg/dL (0.058 nM). This biosensor is highly reproducible even for different concentration levels of creatinine. It is the very first creatinine biosensor possessing a wider linear range for clinical applications for creatinine. To ensure its clinical application, blood serum samples of people of different age groups were collected from Alpha Hospital and analyzed for creatinine by using our chemodosimeter method and compared with data obtained using a commercial method in the Alpha hospital. Our data show very good agreement with clinical data. Because clinical protocol involves trienzy...Continue Reading

References

Mar 15, 1991·Analytical Chemistry·V K NguyenJ P Schwing
Oct 14, 2004·Journal of the American Chemical Society·Matthew D DisneyPeter H Seeberger
Apr 28, 2005·Biosensors & Bioelectronics·John C PickupDavid J S Birch
Mar 23, 2006·Journal of the American Chemical Society·Ghanashyam AcharyaCagri Savran
Dec 2, 2009·Chemistry : a European Journal·Kirill KolmakovStefan W Hell
Apr 30, 2010·Chemical Society Reviews·Zhaochao XuDavid R Spring
Oct 14, 2010·Analytical Chemistry·Maria J Ruedas-Rama, Elizabeth A H Hall
Mar 30, 2011·Chemical Society Reviews·Jiasheng WuPengfei Wang
Oct 4, 2012·Organic & Biomolecular Chemistry·Alexander CiupaLorenzo Caggiano
Nov 21, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Wei WangXiaoyi Xu
Oct 16, 2013·Journal of Fluorescence·Puhui XieLixia Xie
Oct 12, 2015·Analytica Chimica Acta·Bruno DebusAndrey Legin
Jun 25, 2016·Beilstein Journal of Organic Chemistry·Antonia Mielgo, Claudio Palomo
Jul 21, 2017·ACS Sensors·David GaddesSrinivas Tadigadapa

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