Abstract
Miconazole (MIC), an antifungal agent, diplays anti‑tumorigenic activity in various types of human cancers, including bladder cancer, yet its mechanism of antitumor action is not well understood. In the present study, we demonstrated that, in a cell viability assay, MIC had a cytotoxic effect on human T24, J82 and TSGH-8301 bladder cancer cells in a dose- and time‑dependent manner, but did not exhibit significant toxicity toward human peripheral blood mononuclear cells. Cell cycle analysis revealed that MIC at concentrations of 25 and 50 µM significantly caused G0/G1 arrest in the TSGH-8301 and T24 cells, respectively. DNA fragmentation, mitochondrial membrane potential and western blot analyses showed that MIC inhibited the growth of these cells by both mitochondrial‑mediated and death receptor (DR5)‑mediated apoptosis pathways. Specifically, MIC increased the protein levels of p21 and p27, but decreased the expression of cyclin E1, CDK2 and CDK4. MIC augmented the expression of DR5, cleaved forms of caspase-3 -8 and -9, poly(ADP‑ribose) polymerase and Bax, decreased the expression of Bcl-2 but increased cytosol levels of cytochrome c. Our results suggest that MIC inhibits the growth of bladder cancer cells through induction o...Continue Reading
References
Jan 1, 1980·International Review of Cytology·A H WyllieA R Currie
Aug 1, 1996·Current Problems in Cardiology·C V Leier
Jan 9, 1998·Journal of Neurochemistry·T SatohH Hatanaka
Jun 12, 1999·Nature·S ShimizuY Tsujimoto
Feb 8, 2000·Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology·H TaguchiT Yoshida
Mar 7, 2001·Biochemical Pharmacology·Z XiaL Nässberger
Mar 21, 2001·Apoptosis : an International Journal on Programmed Cell Death·H U SimonF Levi-Schaffer
Apr 3, 2001·Nature Cell Biology·R RaviA Bedi
Nov 17, 2001·International Immunopharmacology·O Bruserud
Apr 2, 2002·Toxicology and Applied Pharmacology·Chih-Hsiung WuYuan-Soon Ho
Oct 22, 2002·Urological Research·Sheau-Yun YuanChi-Rei Yang
Jan 28, 2003·Journal of Cellular Physiology·Karyn van de MarkDouglas V Faller
Jun 10, 2004·Biological & Pharmaceutical Bulletin·Yeun-Ja MunWon-Hong Woo
Aug 24, 2004·The Journal of Biological Chemistry·Hirohito Yamaguchi, Hong-Gang Wang
Apr 14, 2005·Life Sciences·Hong-Tai ChangChung-Ren Jan
May 19, 2005·Cancer Research·Andrei L Gartel, Senthil K Radhakrishnan
Jun 21, 2005·Molecular and Cellular Biology·Shashirekha ShettySpencer B Gibson
Jul 5, 2005·Cancer Research·Tatsushi YoshidaToshiyuki Sakai
Nov 3, 2005·Mycoses·U NawrotB Gusin
Oct 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stavroula BaritakiBenjamin Bonavida
Feb 6, 2009·Journal of Cell Science·Joslyn K Brunelle, Anthony Letai
Feb 21, 2009·Cell Death and Differentiation·D SohnR U Jänicke
Aug 20, 2009·BJU International·Pierre ColinMorgan Rouprêt
Sep 29, 2009·The Journal of Biological Chemistry·Isabelle E J A FrançoisKarin Thevissen
Nov 4, 2010·The Journal of Biological Chemistry·You-Take OhShi-Yong Sun
Jul 16, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Chieh-Shan WuChi-Chen Lin
May 10, 2012·Expert Opinion on Pharmacotherapy·Gérald E PiérardClaudine Piérard-Franchimont
May 12, 2012·Journal of Asian Natural Products Research·Hai-Tao ZhangHui Luo
Jun 22, 2012·PloS One·Hui LiYanyan Bian
Sep 6, 2012·Journal of Agricultural and Food Chemistry·Shao-Bin ChengChun-Chi Wu
Feb 12, 2013·Journal of Translational Medicine·Fen YangXiao-Feng Zhu
Sep 27, 2014·Acta histochemica·Agnieszka ZuryńAlina Grzanka
Dec 3, 2014·Biochemical and Biophysical Research Communications·Haihe WangGuofu Chen
Jan 13, 2015·Journal of Cancer Research and Therapeutics·Amir Ali ShahbazfarAfsaneh Khoshkerdar
Feb 13, 2015·Journal of Clinical and Experimental Dentistry·Carla Garcia-CuestaJose V Bagán
Jun 19, 2015·European Journal of Pharmacology·Sheau-Yun YuanChi-Chen Lin
Aug 20, 2015·Anti-cancer Agents in Medicinal Chemistry·Ramandeep KaurVinod Kumar
Nov 30, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sung-Chul LimSong Iy Han
Citations
Jun 2, 2020·Cancer Science·Sung-Hoon YoonMisun Won
Mar 22, 2019·Molecular Medicine Reports·Jie LiGang Chen
May 30, 2019·Cell Death & Disease·Hong-En YuHui-Yan Luo
Feb 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tomhiro MastuoHideki Sakai
Apr 3, 2019·Cancers·Daniela D'ArcangeloAntonio Facchiano
Sep 27, 2018·Cancers·Kohei MoritaChiho Ohbayashi
Sep 28, 2020·Environmental Toxicology·Chao-Yen HoThomas I-Sheng Hwang
Apr 27, 2019·Frontiers in Oncology·Julieti Huch BussFabiana Kömmling Seixas
Oct 6, 2018·European Journal of Pharmacology·Chen-Li ChengSheau-Yun Yuan
Jul 3, 2021·Journal of Fungi·Anne Caroline Morais CaldeirãoDouglas Roberto Monteiro
Nov 9, 2021·Frontiers in Pharmacology·Bo-Young KimKoanhoi Kim