Micro- and nanofabrication of robust reactive arrays based on the covalent coupling of dendrimers to activated monolayers

Langmuir : the ACS Journal of Surfaces and Colloids
Geerten H DegenhartG Julius Vancso

Abstract

We report on methods to fabricate robust micro- and nanopatterned platforms, comprising high functional group densities and quasi three-dimensional structures, for possible applications in biochip array technologies. For this purpose, amine-terminated poly(amidoamine) (PAMAM) dendrimers were immobilized via amide linkage formation on 11,11'-dithiobis(N-hydroxysuccinimidylundecanoate) (NHS-C10) self-assembled monolayers (SAMs) on gold surfaces. The coupling reaction and the resulting assemblies were characterized by grazing incidence reflection Fourier transform infrared spectroscopy, contact angle measurements, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy; the obtained surface coverage values were successfully fitted with a Langmuir isotherm. The fraction of unreacted peripheral primary amine groups of the surface-immobilized PAMAM dendrimers was 28% as determined by XPS analysis of trifluoroacetic anhydride-labeled assemblies. Patterning of the PAMAM dendrimers on NHS-C10 SAMs on the micrometer and sub-100-nm scale was achieved by microcontact printing and dip pen nanolithography. The resulting patterns are characterized by their high degree of order and stability of the transferred molecules due to cova...Continue Reading

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Citations

Feb 8, 2005·Analytical and Bioanalytical Chemistry·Arjan P QuistSven Oscarsson
Aug 23, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Cuie WangShou-Jun Xiao
Jul 26, 2008·Nature Nanotechnology·Khalid SalaitaChad A Mirkin
Jun 12, 2010·Chemical Communications : Chem Comm·Matthew J GreaneyLawrence D Margerum
Feb 22, 2012·Nanoscale·Mianqi XueTingbing Cao
Mar 1, 2012·Analytica Chimica Acta·Admira BosnjakovicRangaramanujam M Kannan
Apr 22, 2005·Chemistry : a European Journal·Christiaan M BruininkDavid N Reinhoudt
May 1, 2016·Colloids and Surfaces. B, Biointerfaces·Ousmane M BaArnaud Ponche
Dec 23, 2006·Physical Chemistry Chemical Physics : PCCP·Michael Woodson, Jie Liu
Apr 2, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Madhukar B KolliEric R Blough
Feb 23, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Mandy YangHua-Zhong Yu
Jul 20, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Robert SchlapakStefan Howorka
Nov 15, 2005·Biomacromolecules·Chuan Liang FengHolger Schönherr
Mar 29, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Parayil Kumaran AjikumarHeng-Phon Too
Aug 24, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Ronald ZirbsWolfgang H Binder
Jan 24, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Chuan Liang FengHolger Schönherr

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