Micro-CT evaluation of rheumatoid arthritis mouse model disease progression: Manual tracings versus semi-automated routines

Diagnostic and Interventional Imaging
A C AntillStephen J Lokitz

Abstract

The primary goal of this study was to demonstrate the value of micro-CT imaging in a rheumatoid arthritis (RA) mouse model. The secondary goal was to assess whether manual correction of the articular surface regions of interest (ROI) identification of the semi-automated methods may result in more effective assessment of bone volume and density loss. Collagen-induced arthritis (CIA) was induced in six DBA/1J mice at 12 weeks of age and three other DBA/1J identical mice served as controls. Micro-CT images were acquired at baseline and at four, seven, and nine weeks post-induction. Disease was monitored via ROI analysis, and ROIs were first generated using semi-automated techniques. These ROIs were manually manipulated so that a variety of edge irregularities were corrected. Effort was focused on the proximal and distal humerus and the distal femur. ROI volume and density were calculated, and data were compared. A histologic analysis of the study mice was also performed after the last time frame. There was a significant difference between the volume data comparison between the manually manipulated data and the semi-automated routine data across all time frames and across both humeri and femurs. There was no significant difference ...Continue Reading

Citations

Mar 16, 2017·Scientific Reports·Carl-Magnus SvenssonHans Peter Saluz
Jan 5, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Kangsheng LiaoTing Li

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