Micro-electrode measurement of skin pH in humans during ischaemia, hypoxia and local hypothermia

The Journal of Physiology
D K Harrison, W F Walker

Abstract

1. The extracellular pH value in the dermis of human skin (skin pH) was measured in vivo using glass micro-electrodes. They were found to be both reliable and accurate. 2. The mean value of skin pH measured in the legs of forty different volunteers was found to be pH 7.54 +/- 0.09 (S.D.). No difference in skin pH was observed between males and females, or in different regions of the limb. 3. Local reductions in skin surface temperature in ten subjects caused an increase of pH 0.023 +/- 0.007 per degree C fall. 4. A 20 min period of tourniquet ischaemia in twenty volunteers induced a fall in skin pH of 0.13 +/- 0.05 units. 5. Hyperventilation during a 10 min period of breathing 10% O2 in N2 caused an increase of pH 0.04 +/- 0.02 in the skin of healthy subjects. 6. Skin pH fell to a value 0.02 +/- 0.02 units below normal 10 min after the hypoxic period, suggesting the presence of excess lactate. 7. Skin pH results compared well with blood gases and pH values of arterialized samples taken during hypoxia. 8. It was concluded that the system was suitable for clinical trials.

References

Jan 1, 1980·Journal of Medical Engineering & Technology·D K Harrison, W F Walker
Jul 3, 2015·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·W LiuW W Lu
Jan 1, 1990·International Journal of Radiation Oncology, Biology, Physics·S A van de MerweH S Reinhold
Aug 26, 2009·Journal of the European Academy of Dermatology and Venereology : JEADV·S SchremlP Babilas
May 1, 1982·European Journal of Cancer & Clinical Oncology·A P van den BergH S Reinhold
Dec 1, 1995·Prosthetics and Orthotics International·D K HarrisonA S Jain
Feb 20, 2013·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Y E Yun, A N Edginton

Related Concepts

Ischemia
In Vivo
Microelectrodes
Dioxygen
Lactate
Extracellular
Human Volunteers
Dermis
Blood Gas
Regional Blood Flow

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