Micro-environmental control of cell migration--myosin IIA is required for efficient migration in fibrillar environments through control of cell adhesion dynamics.

Journal of Cell Science
Andrew D DoyleKenneth M Yamada

Abstract

Recent evidence suggests that organization of the extracellular matrix (ECM) into aligned fibrils or fibril-like ECM topographies promotes rapid migration in fibroblasts. However, the mechanisms of cell migration that are altered by these changes in micro-environmental topography remain unknown. Here, using 1D fibrillar migration as a model system for oriented fibrillar 3D matrices, we find that fibroblast leading-edge dynamics are enhanced by 1D fibrillar micropatterns and demonstrate a dependence on the spatial positioning of cell adhesions. Although 1D, 2D and 3D matrix adhesions have similar assembly kinetics, both 1D and 3D adhesions are stabilized for prolonged periods, whereas both paxillin and vinculin show slower turnover rates in 1D adhesions. Moreover, actin in 1D adhesions undergoes slower retrograde flow than the actin that is present in 2D lamellipodia. These data suggest an increase in mechanical coupling between adhesions and protrusive machinery. Experimental reduction of contractility resulted in the loss of 1D adhesion structure and stability, with scattered small and unstable adhesions, and an uncoupling of adhesion protein-integrin stability. Genetic ablation of myosin IIA (MIIA) or myosin IIB (MIIB) isofor...Continue Reading

References

Nov 27, 2001·Science·E CukiermanK M Yamada
Dec 6, 2003·Science·Anne J RidleyAlan Rick Horwitz
Sep 1, 2005·The Journal of Cell Biology·Roumen PankovKenneth M Yamada
Jan 6, 2007·Science·Ke HuClare M Waterman-Storer
Feb 21, 2007·Nature Cell Biology·Sharona Even-RamKenneth M Yamada
Feb 22, 2007·The Journal of Cell Biology·Miguel Vicente-ManzanaresAlan F Horwitz
Dec 7, 2007·The Journal of Cell Biology·Jonathan D HumphriesChristoph Ballestrem
Jan 30, 2008·Current Protocols in Cell Biology·Erik L SnappJennifer Lippincott-Schwartz
Dec 17, 2008·PloS One·Satoshi HabuchiAntoine M van Oijen
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jordan JacobelliMatthew F Krummel
Feb 18, 2009·The Journal of Cell Biology·Andrew D DoyleKenneth M Yamada
Jul 16, 2009·Nature Reviews. Molecular Cell Biology·Ryan J PetrieKenneth M Yamada
Aug 20, 2009·Cell Adhesion & Migration·Paolo P Provenzano, Patricia J Keely
Dec 3, 2009·The Journal of Cell Biology·Peter Friedl, Katarina Wolf
Dec 17, 2009·Current Protocols in Cell Biology·Andrew D Doyle
Aug 24, 2010·Nature Reviews. Molecular Cell Biology·J Thomas ParsonsMartin A Schwartz
Nov 3, 2010·The Journal of Cell Biology·Scott D Hansen, R Dyche Mullins
Nov 26, 2010·Nature·Pakorn KanchanawongClare M Waterman
Mar 23, 2011·Nature Cell Biology·Dylan T BurnetteJennifer Lippincott-Schwartz
Apr 14, 2011·Journal of Cell Science·Haguy WolfensonBenjamin Geiger
Jun 28, 2011·Cell·Anne J Ridley

❮ Previous
Next ❯

Citations

Jun 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Amit Pathak, Sanjay Kumar
Feb 5, 2013·Journal of Cell Science·Ryan J Petrie, Kenneth M Yamada
Aug 12, 2015·The Journal of Cell Biology·Dustin G ThomasThomas T Egelhoff
Feb 24, 2016·Scientific Reports·Yu-Ja HuangPeter Searson
Jun 16, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Huichang GaoYingjun Wang
Oct 7, 2015·Trends in Cell Biology·Ryan J Petrie, Kenneth M Yamada
Jan 30, 2015·Scientific Reports·Joshua A BroussardDonna J Webb
Nov 11, 2015·The Journal of Cell Biology·Charlotte Guetta-TerrierNils C Gauthier
Mar 24, 2015·Cell·John R DavisBrian M Stramer
Apr 16, 2016·Current Opinion in Cell Biology·Ryan J Petrie, Kenneth M Yamada
Feb 26, 2016·Molecular Biology of the Cell·Pascale MonzoMichael P Sheetz
Jul 4, 2015·Trends in Cell Biology·Anna HaegerPeter Friedl
Jul 16, 2013·Current Opinion in Cell Biology·Andrew D DoyleKenneth M Yamada
Aug 13, 2013·Current Biology : CB·Kristopher E KubowA Rick Horwitz
Aug 1, 2013·Biomaterials·Alexandre LeclercAndrew E Pelling
Jun 12, 2013·Experimental Cell Research·Matthew L KutysKenneth M Yamada
Nov 3, 2015·Experimental Cell Research·Andrew D Doyle, Kenneth M Yamada
Apr 28, 2016·Biophysical Journal·Daniel F MilanoAnand R Asthagiri
Oct 21, 2014·PloS One·Annika KastenRolf E Brenner
Jul 16, 2016·Annual Review of Biomedical Engineering·Colin D PaulKonstantinos Konstantopoulos
Sep 3, 2016·Cell Adhesion & Migration·Sangyoon J HanNathan J Sniadecki
Sep 16, 2016·PloS One·Yi Wen ChaiJohn H Brekke
Feb 24, 2017·Molecular Biology of the Cell·G R Ramirez-San JuanM L Gardel
Mar 15, 2018·Journal of Cell Science·Neha SaxenaShamik Sen
Sep 17, 2016·F1000Research·Stacey Lee, Sanjay Kumar
Nov 8, 2018·Annual Review of Physiology·Haguy WolfensonMichael P Sheetz
Mar 5, 2019·Cellular and Molecular Life Sciences : CMLS·Katharina VestreCinzia Progida
Jan 29, 2014·Physical Chemistry Chemical Physics : PCCP·Vesa P Hytönen, Bernhard Wehrle-Haller
Oct 5, 2019·Nature Reviews. Molecular Cell Biology·Kenneth M Yamada, Michael Sixt
Dec 24, 2019·Advanced Healthcare Materials·Kwang Hoon SongJason A Burdick
Dec 10, 2019·Advanced Healthcare Materials·Marco CantiniManuel Salmeron-Sanchez
May 12, 2020·Frontiers in Physiology·Mary T DoolinKimberly M Stroka
Jul 31, 2020·Molecular Biology of the Cell·Vivian W Tang
Jan 6, 2017·The Journal of Biological Chemistry·Vandana RaiThomas T Egelhoff
Jul 9, 2017·The Journal of Cell Biology·Bruno PontesNils C Gauthier
May 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shichen ZhuYang Hu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Biophysics of Adhesion

Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.