Micro free flow electrophoresis

Lab on a Chip
Alexander C Johnson, Michael T Bowser

Abstract

Micro free-flow electrophoresis (μFFE) is a continuous separation technique in which analytes are streamed through a perpendicularly applied electric field in a planar separation channel. Analyte streams are deflected laterally based on their electrophoretic mobilities as they flow through the separation channel. A number of μFFE separation modes have been demonstrated, including free zone (FZ), micellar electrokinetic chromatography (MEKC), isoelectric focusing (IEF) and isotachophoresis (ITP). Approximately 60 articles have been published since the first μFFE device was fabricated in 1994. We anticipate that recent advances in device design, detection, and fabrication, will allow μFFE to be applied to a much wider range of applications. Applications particularly well suited for μFFE analysis include continuous, real time monitoring and microscale purifications.

References

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Citations

Jan 28, 2020·Electrophoresis·Tristan F KindeDebashis Dutta
Jun 27, 2020·Lab on a Chip·Tom ScheidtTuomas P J Knowles
Jun 6, 2021·Journal of Chromatography. a·Lily FarmerieMohamed Dawod
Jul 14, 2021·Analytical Chemistry·Sven KochmannSergey N Krylov
Apr 28, 2020·Analytical Chemistry·Matthew CourtneyCarolyn L Ren
Jul 4, 2018·Analytical Chemistry·Sven Kochmann, Sergey N Krylov
Jan 29, 2020·Analytical Chemistry·Nikita A IvanovSergey N Krylov
Oct 23, 2021·Electrophoresis·Matthew CourtneyCarolyn L Ren

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Methods Mentioned

BETA
electrophoresis
chip
biosensors
surface
fluorescence imaging
CE-SELEX

Software Mentioned

μFFE

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