Micro-heterogeneity and aggregation in beta2-microglobulin solutions: effects of temperature, pH, and conformational variant addition

European Biophysics Journal : EBJ
Roberto PiazzaVittorio Bellotti

Abstract

We show that beta(2)-microglobulin solutions in physiological conditions contain a tiny fraction of aggregates, which can hardly be filtered out and tend to re-form spontaneously. At physiological pH the fractional amount and size distribution of the latter aggregates do not depend on temperature. Conversely, in the pH range typical of the peri-articular tissue acidosis that often occurs in hemodialysis, temperature increase leads to fast and irreversible growth of the aggregates. Quite similar, but strongly enhanced aggregation effects can be induced even in physiological conditions by adding a very small amount of DeltaN6, a naturally occurring truncated isoform of beta(2)-m known to promote fibrillogenesis.

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Citations

Jan 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Katarzyna DomanskaJan Steyaert
Nov 15, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Sofia GiorgettiVittorio Bellotti
Apr 22, 2009·Journal of Molecular Biology·Kenji SasaharaYuji Goto
Jun 1, 2017·Metallomics : Integrated Biometal Science·Sarah Ameziane-Le HirClaude Vidaud
Jan 1, 2013·Biomolecules·Annalisa ReliniAlessandra Gliozzi
Dec 7, 2007·The Journal of Biological Chemistry·Annalisa ReliniVittorio Bellotti
May 21, 2011·The FEBS Journal·Timo Eichner, Sheena E Radford
May 28, 2010·Protein Science : a Publication of the Protein Society·Carlo SantambrogioRita Grandori
Mar 10, 2015·The Journal of Biological Chemistry·Monica Stoppini, Vittorio Bellotti
Feb 28, 2016·The Journal of Biological Chemistry·Antonino NatalelloSara Raimondi
Nov 3, 2020·Frontiers in Molecular Biosciences·Rui J S Loureiro, Patrícia F N Faísca

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