Micro-injector for capillary electrophoresis

Electrophoresis
Jorge SáizPeter C Hauser

Abstract

A novel micro-injector for capillary electrophoresis for the handling of samples with volumes down to as little as 300 nL was designed and built in our laboratory for analyses in which the available volume is a limitation. The sample is placed into a small cavity located directly in front of the separation capillary, and the injection is then carried out automatically by controlled pressurization of the chamber with compressed air. The system also allows automated flushing of the injection chamber as well as of the capillary. In a trial with a capillary electrophoresis system with contactless conductivity detector, employing a capillary of 25 μm diameter, the results showed good stability of migration times and peak areas. To illustrate the technique, the fast separation of five inorganic cations (Na(+) , K(+) , NH4 (+) , Ca(2+) , and Mg(2+) ) was set up. This could be achieved in less than 3 min, with good limits of detection (10 μM) and linear ranges (between about 10 and 1000 μM). The system was demonstrated for the determination of the inorganic cations in porewater samples of a lake sediment core.

References

Oct 31, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Gamze Kavran BelinPeter C Hauser
Mar 26, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Worapan PormsilaPeter C Hauser
Aug 7, 2012·Analytical and Bioanalytical Chemistry·Marco Grundmann, Frank-Michael Matysik
Sep 12, 2012·Electrophoresis·Eric T da CostaClaudimir Lucio do Lago
Sep 25, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Thi Thanh Thuy PhamPeter C Hauser
Mar 16, 2013·Environmental Science. Processes & Impacts·Natascha T TorresBeat Müller
Mar 13, 2014·Environmental Science. Processes & Impacts·Natascha T TorresBeat Müller

❮ Previous
Next ❯

Citations

Jun 6, 2017·Journal of Separation Science·František Opekar, Petr Tůma
Sep 20, 2018·Electrophoresis·Petr KubáňGuillaume Erny
Sep 18, 2020·Analytical Methods : Advancing Methods and Applications·Andreea Oarga MulecRadmila Milačič
Jan 17, 2021·Journal of Chromatography. a·Joshua J DavisJames P Grinias

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.