Micro-method for the determination of piperacillin in plasma by high-performance liquid chromatography

Journal of Pharmaceutical and Biomedical Analysis
V GautierG Vinçon

Abstract

A simple and sensitive high-performance liquid chromatographic method for the determination of piperacillin in plasma is described. A C8 reversed-phase column was used with a mobile phase consisting of methanol-water-triethylamine (550:450:4, v/v/v) adjusted to pH = 3 with orthophosphoric acid and UV detection at 270 nm. Cephalothin was used as internal standard. The method involves a plasma protein precipitation with acetonitrile followed by extraction of endogenous compound with chloroform and injection of the upper aqueous phase into the chromatograph. Within-day and between-day assays give relative standard deviations less than or equal to 5.7%. The detection limit is 0.2 microgram ml-1. Stability studies show that piperacillin degradation starts at -4 degrees C. Therefore, samples have to be processed promptly and stored at -20 degrees C. The method described is convenient for clinical monitoring and for pharmacokinetic studies.

References

Jan 22, 1988·Journal of Chromatography·M A Riegel, P P Ellis
May 10, 1985·Journal of Chromatography·M C Rouan
Jul 12, 1985·Journal of Chromatography·F Demotes-MainardH C Albin
Apr 1, 1982·Antimicrobial Agents and Chemotherapy·A M Brisson, J B Fourtillan
Dec 10, 1982·Journal of Chromatography·M K AravindR E Kauffman

Citations

Oct 16, 2004·Biomedical Chromatography : BMC·Chonghua LiDavid P Nicolau
Jan 1, 1993·European Journal of Clinical Pharmacology·M DuponJ Saric
Feb 24, 2001·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·B TangH Y Wang
Sep 15, 2006·Journal of Separation Science·Victoria F SamanidouIoannis N Papadoyannis

Related Concepts

High Pressure Liquid Chromatography Procedure
Microchemistry
Piperacillin-ratiopharm
Solutions
Spectrophotometry, Ultraviolet

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