Micro-structured, spontaneously eroding hydrogels accelerate endothelialization through presentation of conjugated growth factors

Biomaterials
Bettina E B JensenAlexander N Zelikin

Abstract

Growth factors represent highly potent and highly efficacious means of communication to cells. At the same time, these proteins are fragile and relatively small sized--rendering their immobilization and controlled release from biomaterials challenging. In this work, we establish a method to incorporate growth factors into the physical hydrogels based on poly(vinyl alcohol), PVA. The latter have a long and successful history of biomedical applications and approval for diverse use in human patients, but are also characterized with scant opportunities for bioconjugation and functionalization. Herein, we develop the conjugation of growth factors to the micro-structured, spontaneously eroding physical hydrogels based on PVA. Protein conjugation was elaborated using model substrates, albumin and lysozyme, which aided to reveal specificity of chemical reactions and benign, non-harmful nature of the established protocols. Surface-adhered format of hydrogel analyses allowed to quantify bioconjugation reactions and enzymatic activity of the immobilized proteins and to visualize the hydrogels with immobilized cargo. In cell culture, immobilized growth factors were effective in communicating to adhering cells and specifically enhanced prol...Continue Reading

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Citations

Mar 10, 2016·The Journal of Physical Chemistry. B·Bettina E B JensenAlexander N Zelikin
Oct 22, 2016·Nature Chemistry·Alexander N ZelikinAnne Marie Healy
Jul 9, 2020·Frontiers in Bioengineering and Biotechnology·Daniela Enriquez-OchoaMarion E G Brunck
Jan 23, 2021·International Journal of Molecular Sciences·Érica Resende OliveiraAmin Shavandi
Nov 23, 2018·Acta Biomaterialia·Cristobal Garcia Garcia, Kristi L Kiick
Jul 3, 2020·ACS Nano·Søren L PedersenAlexander N Zelikin
Aug 12, 2019·ACS Biomaterials Science & Engineering·Miyeon NohNathaniel S Hwang

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