Micro-vesicles from mesenchymal stem cells over-expressing miR-34a inhibit transforming growth factor-β1-induced epithelial-mesenchymal transition in renal tubular epithelial cells in vitro.

Chinese Medical Journal
Juan HeShi-Ren Sun

Abstract

The use of microRNAs in the therapy of kidney disease is hampered by the difficulties in their effective delivery. Micro-vesicles (MVs) are known as natural carriers of small RNAs. Our prior research has demonstrated that MVs isolated from mesenchymal stem cells (MSCs) are capable of attenuating kidney injuries induced by unilateral ureteral obstruction and 5/6 sub-total nephrectomy in mice. The present study aimed to evaluate the effects of miR-34a-5p (miR-34a)-modified MSC-MVs on transforming growth factor (TGF)-β1-induced fibrosis and apoptosis in vitro. Bone marrow MSCs were modified by lentiviruses over-expressing miR-34a, from which MVs were collected for the treatment of human Kidney-2 (HK-2) renal tubular cells exposed to TGF-β1 (6 ng/mL). The survival of HK-2 cells was determined using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) and Annexin V-Light 650/propidium iodide (PI) assays. The expression levels of epithelial markers (tight junction protein 1 [TJP1] and E-cadherin) and mesenchymal markers (smooth muscle actin alpha (α-SMA) and fibronectin) in HK-2 cells were measured using Western blot analysis and an immunofluorescence assay. In addition, changes in Notch-1/Jagged-1 signaling were anal...Continue Reading

References

Jun 1, 2002·Journal of the American Society of Nephrology : JASN·Jeremiah MorrisseySaulo Klahr
Jan 1, 2004·Genes & Development·Luika A TimmermanJosé Luis de la Pompa
Jan 10, 2009·Nature Protocols·Masoud Soleimani, Samad Nadri
Dec 1, 2009·Cell·Jean Paul ThieryM Angela Nieto
Jun 23, 2010·Human Gene Therapy·Pierre Charbord
Mar 4, 2011·The Journal of Clinical Investigation·Wilhelm KrizMichel Le Hir
Feb 14, 2012·Seminars in Cell & Developmental Biology·Chandramouli R ChillakuriPenny A Handford
May 10, 2012·The Journal of Pathology·Marco PrunottoSolange Moll
Apr 25, 2013·Cancer Letters·Pierre GalichonAlexandre Hertig
Aug 12, 2014·Journal of Cell Science·Yang ZhouJunwei Yang
Nov 12, 2014·Nature Reviews. Nephrology·Piera TrionfiniGiuseppe Remuzzi
Oct 9, 2015·Stem Cell Research & Therapy·Fengyun ZhangBo Yu
May 21, 2016·Molecular Therapy : the Journal of the American Society of Gene Therapy·Bo WangSharon D Ricardo
Jan 25, 2017·Nature·Xianchi DongTimothy A Springer
Feb 17, 2017·Pharmacology & Therapeutics·Lucio Barile, Giuseppe Vassalli
Feb 22, 2017·Advances in Protein Chemistry and Structural Biology·J YangA R Brasier
Apr 14, 2017·Current Gene Therapy·Arash Aghajani NargesiAlfonso Eirin
Nov 9, 2017·Annals of Internal Medicine·Andrew S Levey, Matthew T James
Dec 2, 2017·Cellular Signalling·Stephen P HigginsPaul J Higgins
Dec 2, 2017·Journal of the American Society of Nephrology : JASN·Norberto PericoGiuseppe Remuzzi
Mar 12, 2019·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yong LiuJinghong Zhao
Jul 12, 2019·Advanced Drug Delivery Reviews·Alicia Fernández-ColinoPetra Mela
Jul 13, 2019·Nature Reviews. Nephrology·Yuka SatoRichard J Johnson

❮ Previous
Next ❯

Citations

Jun 3, 2021·International Journal of Molecular Sciences·Raphael Rodrigues CorrêaRafael Soares Lindoso

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
transmission electron microscopy
PCR
electrophoresis
electron microscopy
genetic modification

Software Mentioned

GraphPad
GraphPad Prism

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Alagille Syndrome

Alagille syndrome is a multi-system genetic disorder that can affect the liver, heart, and other parts of the body. It’s characterization includes heart problems, liver damage, jaundice, and xanthomas. Here is the latest research.