Microalgal Microscale Model for Microalgal Growth Inhibition Evaluation of Marine Natural Products

Scientific Reports
Qing ZhaoChang-Yun Wang

Abstract

Marine organisms especially sessile invertebrates, such as soft corals, gorgonians and sponges, can survive in the competitive environment mainly relying on their second metabolites with chemoecological effects including allelopathy and algal growth inhibition. It is well known that the microscale models are urgently needed in marine chemoecology assessment to evaluate the algal growth inhibition activity of trace quantity natural products. In this work, a microalgal growth inhibition model was established for microalgal inhibition evaluation of marine natural products with 96-well microplate by automatic fluorescence observation using microplate reader. Subsequently, this model was applied to bioassay-guided isolation and preliminary bioactivity screening of the secondary metabolites from soft corals, gorgonians, sponges and their symbiotic microbes collected from the South China Sea. As a result, fifteen compounds (1‒15) were found to exhibit microalgal growth inhibition activities against at least one of marine microalgae, Karenia mikimotoi, Isochrysis galbana, and Heterosigma akashiwo. Specifically, altersolanol C (13) demonstrated potent activity against K. mikimotoi with the 96h-EC50 value of 1.16 µg/mL, more than four ti...Continue Reading

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Citations

Aug 11, 2020·Heliyon·Kasturi ArumugamVictor S Kuwahara
Apr 4, 2020·Journal of Natural Products·Yun-Feng LiuChang-Yun Wang

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Methods Mentioned

BETA
column chromatography
silica gel column chromatography
X-ray

Software Mentioned

Excel
PROBIT
SPSS

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