Microanalysis of glycosaminoglycan-derived disaccharides labeled with the fluorophore 2-aminoacridone by capillary electrophoresis and high-performance liquid chromatography

Analytical Biochemistry
Hiroshi KitagawaK Sugahara

Abstract

A series of disaccharides derived from chondroitin sulfate and heparin/heparan sulfate were derivatized at their reducing ends with the fluorophore 2-aminoacridone. The resulting labeled compounds derived from chondroitin sulfate or heparin/heparan sulfate were well separated and could be quantified by capillary electrophoresis and HPLC with a fluorescence detector. This method was successfully applied to the analysis of the disaccharide composition of sulfated tetra- and hexasaccharides derived from chondroitin sulfate and heparin/heparan sulfate. Remarkably, the saturated and the corresponding unsaturated chondrodisaccharides, the structures of which differ only in their nonreducing terminal uronic acid residues, were also well separated from each other by capillary electrophoresis. This facilitated disaccharide composition analysis of saturated chondrooligosaccharides prepared by testicular hyaluronidase digestion as well as unsaturated chondro- and heparin/heparan sulfate-oligosaccharides prepared by digestion with chondroitinases and heparitinases. The developed method employing the derivatization by 2-aminoacridone in conjunction with capillary electrophoresis or HPLC with a fluorescence detector allows a simple, sensitiv...Continue Reading

Citations

Sep 21, 2010·Analytical and Bioanalytical Chemistry·Bo YangRobert J Linhardt
Nov 14, 2013·Nature Communications·Kosei TakeuchiMichihiro Igarashi
Jan 17, 2012·Nature Neuroscience·Shinji MiyataHiroshi Kitagawa
Jul 15, 2009·The Journal of Biological Chemistry·Scott E GuimondJeremy E Turnbull
Dec 3, 2010·The Journal of Biological Chemistry·Satomi NadanakaHiroshi Kitagawa
Dec 12, 2013·Glycobiology·Leyla GasimliRobert J Linhardt
Dec 15, 2015·Glycoconjugate Journal·Ebru UcakturkRobert J Linhardt
Feb 3, 2011·Biochemical and Biophysical Research Communications·Vita Yanti AnggraeniKen-ichi Hirata
Aug 19, 2015·Experimental Neurology·Patrice D SmithJames W Fawcett
Jan 5, 2011·Biomedical Chromatography : BMC·Christina J MalavakiNikos K Karamanos
Jun 7, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Nicola Volpi, Francesca Maccari
Jun 24, 2008·Analytical Biochemistry·J Timothy King, Umesh R Desai
Apr 10, 2010·The Biochemical Journal·Megumi OkadaHiroshi Kitagawa
Jun 10, 2011·Communicative & Integrative Biology·Shuhei YamadaSuat Ozbek
Jan 25, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mitsuhiro Kinoshita, Kazuaki Kakehi
May 13, 2014·Analytical and Bioanalytical Chemistry·Ebru UcakturkRobert J Linhardt
Feb 22, 2002·Biomedical Chromatography : BMC·Wenjun MaoRobert J Linhardt
Jan 1, 2012·Metabolites·Tania M Puvirajesinghe, Jeremy E Turnbull
Jul 18, 2008·Electrophoresis·Nicola VolpiRobert J Linhardt
Nov 28, 2008·Electrophoresis·Alicia M HitchcockJoseph Zaia
Dec 16, 1998·Electrophoresis·S Suzuki, S Honda
Oct 7, 2017·Current Medicinal Chemistry·Radosław SadowskiBogusław Buszewski
Jan 18, 2020·Journal of Biochemistry·Keiko Akasaka-ManyaTamao Endo
Jan 23, 2016·Journal of Separation Science·Jennifer M Fasciano, Neil D Danielson
Jan 1, 2013·Biomolecules·Jun-Ichi FurukawaYasuro Shinohara
Feb 11, 2014·The Journal of Biological Chemistry·Eric P SchmidtRobert J Linhardt
Feb 20, 2018·Frontiers in Integrative Neuroscience·Shinji MiyataHiroshi Kitagawa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.