Microarray analysis of equine endometrium at days 8 and 12 of pregnancy

Biology of Reproduction
M MerklStefan Bauersachs

Abstract

Establishment and maintenance of pregnancy in equids is only partially understood. To provide new insights into early events of this process, we performed a systematic analysis of transcriptome changes in the endometrium at Days 8 and 12 of pregnancy. Endometrial biopsy samples from pregnant and nonpregnant stages were taken from the same mares. Composition of the collected biopsy samples was analyzed using quantitative stereological techniques to determine proportions of surface and glandular epithelium and blood vessels. Microarray analysis did not reveal detectable changes in gene expression at Day 8, whereas at Day 12 of pregnancy 374 differentially expressed genes were identified, 332 with higher and 42 with lower transcript levels in pregnant endometrium. Expression of selected genes was validated by quantitative real-time RT-PCR. Gene set enrichment analysis, functional annotation clustering, and cocitation analysis were performed to characterize the genes differentially expressed in Day 12 pregnant endometrium. Many known estrogen-induced genes and genes involved in regulation of estrogen signaling were found, but also genes known to be regulated by progesterone and prostaglandin E2. Additionally, differential expressio...Continue Reading

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Datasets Mentioned

BETA
GSE21046

Methods Mentioned

BETA
biopsy
sedation
Feature Extraction
PCR
GTPase
biopsies

Software Mentioned

vsn
SAM
Ensembl
LightCycler
Pathway Architect
Feature Extraction
GSEA
DAVID Functional Annotation Clustering
Entrez
newCAST

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