Microarray analysis of long non-coding RNA expression profiles in monocytic myeloid-derived suppressor cells in Echinococcus granulosus-infected mice

Parasites & Vectors
Aiping YuYujuan Shen

Abstract

Cystic echinococcosis is a worldwide chronic zoonotic disease caused by infection with the larval stage of Echinococcus granulosus. Previously, we found significant accumulation of myeloid-derived suppressor cells (MDSCs) in E. granulosus infection mouse models and that they play a key role in immunosuppressing T lymphocytes. Here, we compared the long non-coding RNA (lncRNA) and mRNA expression patterns between the splenic monocytic MDSCs (M-MDSCs) of E. granulosus protoscoleces-infected mice and normal mice using microarray analysis. LncRNA functions were predicted using Gene Ontology enrichment and the Kyoto Encyclopedia of Genes and Genomes pathway analysis. Cis- and trans-regulation analyses revealed potential relationships between the lncRNAs and their target genes or related transcription factors. We found that 649 lncRNAs were differentially expressed (fold change ≥ 2, P < 0.05): 582 lncRNAs were upregulated and 67 lncRNAs were downregulated; respectively, 28 upregulated mRNAs and 1043 downregulated mRNAs were differentially expressed. The microarray data was validated by quantitative reverse transcription-PCR. The results indicated that mRNAs co-expressed with the lncRNAs are mainly involved in regulating the actin cyt...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 24, 2006·Biochimica Et Biophysica Acta·Hideki Yamaguchi, John Condeelis
Mar 28, 2008·Immunological Reviews·Ilaria MarigoVincenzo Bronte
Oct 4, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Je-In YounDmitry I Gabrilovich
Apr 4, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Suzanne Ostrand-Rosenberg, Pratima Sinha
Nov 3, 2009·The Journal of Pathology·Ryan J TaftJohn S Mattick
Nov 27, 2009·European Journal of Immunology·Luigi DolcettiVincenzo Bronte
Jan 14, 2011·Molecular Cancer Research : MCR·Mie IchikawaGeetha Srikrishna
Sep 20, 2011·Molecular Cell·Kevin C Wang, Howard Y Chang
Feb 18, 2012·Nature·Mitchell Guttman, John L Rinn
May 2, 2012·Cancer Immunology, Immunotherapy : CII·Suzanne Ostrand-RosenbergChristopher Ecker
Jun 6, 2012·Annual Review of Biochemistry·John L Rinn, Howard Y Chang
Jun 13, 2012·BMJ : British Medical Journal·Donald P McManusYurong Yang
Apr 3, 2013·The American Journal of Tropical Medicine and Hygiene·Christine M BudkeMen-Bao Qian
Jul 6, 2013·Cell·Igor Ulitsky, David P Bartel
Sep 26, 2013·Nature Reviews. Cancer·James E Talmadge, Dmitry I Gabrilovich
Jul 15, 2015·The Journal of Clinical Investigation·Douglas Marvel, Dmitry I Gabrilovich
Aug 5, 2015·Virus Research·Buhle MoyoPatrick B Arbuthnot
Sep 1, 2015·Veterinary Parasitology·Philip CraigBelgees Boufana
Dec 17, 2015·World Journal of Hepatology·Yong-Ru LiuGang Chen
Feb 10, 2016·Trends in Immunology·Vinit KumarDmitry I Gabrilovich
Feb 18, 2016·Genomics, Proteomics & Bioinformatics·Yiwen Fang, Melissa J Fullwood
Jul 7, 2016·Nature Communications·Vincenzo BronteDmitry I Gabrilovich
Jul 20, 2016·Immunology and Cell Biology·Clara McClureMohamed El Gazzar
Jul 22, 2017·Acta Tropica·Akira Ito, Christine M Budke
Sep 22, 2017·Molecular Immunology·Jun DaiMohamed El Gazzar

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Methods Mentioned

BETA
Feature Extraction
reverse transcription-PCR

Software Mentioned

TreeView
Feature Extraction
SPSS
Cluster
Cytoscape
GeneSpring

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