Microarray analysis of PDGFR alpha+ populations in ES cell differentiation culture identifies genes involved in differentiation of mesoderm and mesenchyme including ARID3b that is essential for development of embryonic mesenchymal cells

Developmental Biology
Atsushi TakebeShin-ichi Nishikawa

Abstract

An inherent difficulty in using DNA microarray technology on the early mouse embryo is its relatively small size. In this study, we investigated whether use of ES cell differentiation culture, which has no theoretical limit in the number of cells that can be generated, can improve this situation. Seven distinct ES-cell-derived populations were analyzed by DNA microarray and examined for genes whose distribution patterns are similar to those of PDGFRalpha, a gene implicated in differentiation of mesoderm/mesenchymal lineages. Using software developed in our laboratory, we formed a group of 30 genes which showed the highest similarity to PDGFRalpha, 18 of these genes were shown to be involved in development of either mesodermal, mesenchymal or neural crest cells. This list also contains several genes whose role in embryogenesis has not yet been fully identified. One such molecule is mARID3b. The mARID3b expression is found in the paraxial mesoderm and cranial mesenchyme. mARID3b-null mouse showed early embryonic lethality, and most phenotypes of this mutant appear to develop from a failure to generate a sufficient number of cranial mesenchymal cells. These results demonstrate the potential use of ES cell differentiation culture i...Continue Reading

References

Nov 22, 1991·Science·R A Weinberg
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D A StephensonV M Chapman
Dec 15, 1993·Gene·W J Gehring
Oct 1, 1993·Analytical Biochemistry·T YagiS Aizawa
Nov 1, 1996·The Journal of Investigative Dermatology·N TakakuraS I Nishikawa
Jun 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·N TakakuraS Nishikawa
Jan 23, 1999·Nature Genetics·C Debouck, P N Goodfellow
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·T Era, O N Witte
Jun 6, 2000·Trends in Biochemical Sciences·R D KortschakR Saint
Jan 29, 2002·Nature Cell Biology·Daniel S PeeperRené Bernards
Jun 28, 2002·The Journal of Biological Chemistry·Melany JacksonGerard J Graham
Mar 1, 2003·Nature Genetics·Kathryn V Anderson, Philip W Ingham
Apr 2, 2003·Human Molecular Genetics·Lee Smith, Andy Greenfield
Apr 8, 2003·Journal of Cell Science·Naoki NakayamaChun-Ya E Han
Nov 19, 2003·Developmental Biology·David A F LoebelPatrick P L Tam
Dec 31, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·James A WestonShinichi Nishikawa
Nov 17, 2004·Current Pharmaceutical Biotechnology·Eleanor Chen, Stephen C Ekker

❮ Previous
Next ❯

Citations

Apr 8, 2014·Gene·Serene J SamyesudhasKaren D Cowden Dahl
Feb 28, 2008·BMC Developmental Biology·Brendan A S McIntyreGuojun Sheng
May 12, 2009·Nature Genetics·Christopher Newton-ChehPatricia B Munroe
Apr 21, 2011·The Journal of Clinical Investigation·Yoko FukushimaAkiyoshi Uemura
Jan 6, 2012·Orphanet Journal of Rare Diseases·Pilar L Magoulas, Ayman W El-Hattab
Jun 26, 2012·The Journal of Biological Chemistry·Michinori KitagawaTakumi Era
Mar 9, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ioannis A Voutsadakis
Jul 4, 2012·Oncogene·K KobayashiS-I Nishikawa
Feb 4, 2016·Nature Reviews. Molecular Cell Biology·Timo Quante, Adrian Bird
Feb 26, 2008·Cancer Science·Lars Martin Jakt, Shinichi Nishikawa
Sep 17, 2008·Biology of the Cell·Nathalie BillonChristian Dani
Nov 12, 2014·Proceedings of the National Academy of Sciences of the United States of America·Michael J MontagueWesley C Warren
Jun 30, 2015·PloS One·Alexander BobbsKaren D Cowden Dahl
May 18, 2005·Biochemical and Biophysical Research Communications·Ying CaoRobert Chunhua Zhao
Dec 16, 2011·Biomaterials·Sepideh Heydarkhan-HagvallRichard J Shemin
Aug 19, 2016·PloS One·Jeffrey L KurkewichKaren D Cowden Dahl
Jun 22, 2017·Development·Jérome Chal, Olivier Pourquié
Aug 12, 2016·Journal of Virology·Jennifer J WoodDavid J Hughes
Oct 27, 2017·Proceedings of the National Academy of Sciences of the United States of America·Karl B ShpargelTerry Magnuson
Aug 16, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Asghar AliQuinton A Winger
Sep 24, 2010·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Ayman W El-HattabSau Wai Cheung
May 26, 2009·Development·Masaki KinoshitaShin-Ichi Nishikawa
Oct 23, 2014·Development·Verónica UribeJuan José Sanz-Ezquerro
Nov 6, 2014·Development·Ravindra B ChalamalasettyTerry P Yamaguchi
May 1, 2013·Genes & Development·Allan M GurtanPhillip A Sharp

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cardiovascular Homeostasis

Cardiovascular regulation and homeostasis is important in maintaining health and dysfunction may lead to cardiovascular diseases. Nitric oxide and vascular endothelium are one of many molecules involved in the homeostatic mechanism. Here is the latest research on cardiovascular homeostasis.