Microarray analysis of toxicogenomic effects of triclosan on Staphylococcus aureus

Applied Microbiology and Biotechnology
Hyeung-Jin JangWilliam E Bentley

Abstract

For the first time, a genome-wide transcriptional analysis was performed to elucidate the cellular response of Staphylococcus aureus to triclosan. Our results indicate that the effects of triclosan are widespread on metabolism, affecting many vital cellular processes. Triclosan downregulated the transcription of genes involved in virulence factor and energy metabolism such as amino acid, carbohydrate, lipid transport, and metabolism, while multidrug resistance genes, coenzyme transport, and metabolism and transcription genes were upregulated. Furthermore, triclosan downregulated the transcription of genes encoding major lipid metabolism enzymes such as 3-hydroxyacyl-CoA dehydrogenase, acetyl-CoA acetyltransferase, acetyl-CoA synthetase, and acetyl-CoA carboxylase, which all play essential roles in S. aureus lipid metabolism. It is interesting to note that the expression of the enoyl-ACP reductase gene, fabI, was not changed after exposure of S. aureus with 0.05 microM triclosan at 10 and 60 min in our study. This work also implies that triclosan may kill S. aureus by interfering with its ability to form cell membranes. Another important implication of our result is that S. aureus may generate resistance factors under triclosan ...Continue Reading

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Citations

Sep 17, 2009·Applied Microbiology and Biotechnology·Hyeung-Jin JangWilliam E Bentley
Sep 21, 2011·The Journal of Antimicrobial Chemotherapy·Tina Wecke, Thorsten Mascher
Apr 7, 2010·Applied and Environmental Microbiology·Benny F G PyckeNatalie Leys
Oct 14, 2008·BMC Genomics·Chantal W NdeWilliam E Bentley
Dec 3, 2014·The Science of the Total Environment·Lisbeth SchnugHans Petter Leinaas
Mar 16, 2021·Frontiers in Cellular and Infection Microbiology·Ping WangDi Xiao
Apr 21, 2018·Microbiology Spectrum·Jean-Yves Maillard

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