Microarray analysis of transcriptional responses to infection by herpes simplex virus types 1 and 2 and their US3-deficient mutants

Microbes and Infection
Maki KamakuraYukihiro Nishiyama

Abstract

Herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) induce similar responses in infected cells and animals but differ in several significant respects. Previous studies have shown that defects in the US3-encoded protein kinase greatly affect both viruses in their interactions with cells and hosts. To investigate the impact of infection with HSV-1, HSV-2 and their US3-deficient mutants (DeltaUS3) on cellular transcriptional responses, we performed a global microarray analysis on human epithelial HEp-2 cells that were mock-infected, or infected with wild-type (WT) HSV-1, HSV-2 and their DeltaUS3 mutants. Among 54,765 probe sets examined, only 1156 (approximately 2.1%) and 2006 (approximately 3.7%) genes increased by at least fourfold at 9h postinfection in WT HSV-1 and HSV-2-infected cells, respectively. Unexpectedly, HSV-2 infection increases mRNA levels for a larger number of cellular genes than HSV-1 infection. Additionally, DeltaUS3 infection upregulated the expression of a larger number of cellular genes than WT infection. The genes affected by HSV infection were assigned to various groups of functional classes and cellular pathways. We have thus identified cellular genes whose expression was similarly or differently changed b...Continue Reading

References

Oct 1, 1987·The Journal of General Virology·M C FrameD P Leader
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·R LeopardiB Roizman
Feb 13, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T MurataY Nishiyama
Dec 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Brunella TaddeoBernard Roizman
Apr 5, 2005·Nature Reviews. Microbiology·Richard G Jenner, Richard A Young

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Citations

May 1, 2012·Biochimica Et Biophysica Acta·Juliana L AbrantesThiago Moreno L Souza
Jul 21, 2012·The Journal of General Virology·Rachael WashPaul Kellam
Nov 6, 2020·Journal of Virology·Caroline C FriedelLars Dölken

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