Microarray analysis on gene regulation by estrogen, progesterone and tamoxifen in human endometrial stromal cells

International Journal of Molecular Sciences
Chun-E RenShi-Wen Jiang

Abstract

Epithelial stromal cells represent a major cellular component of human uterine endometrium that is subject to tight hormonal regulation. Through cell-cell contacts and/or paracrine mechanisms, stromal cells play a significant role in the malignant transformation of epithelial cells. We isolated stromal cells from normal human endometrium and investigated the morphological and transcriptional changes induced by estrogen, progesterone and tamoxifen. We demonstrated that stromal cells express appreciable levels of estrogen and progesterone receptors and undergo different morphological changes upon hormonal stimulation. Microarray analysis indicated that both estrogen and progesterone induced dramatic alterations in a variety of genes associated with cell structure, transcription, cell cycle, and signaling. However, divergent patterns of changes, and in some genes opposite effects, were observed for the two hormones. A large number of genes are identified as novel targets for hormonal regulation. These hormone-responsive genes may be involved in normal uterine function and the development of endometrial malignancies.

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Citations

Nov 26, 2015·International Journal of Environmental Research and Public Health·Dongoh LeeEui-Bae Jeung
Aug 2, 2017·Reproduction in Domestic Animals = Zuchthygiene·L ZhangB-Y Cao
Apr 9, 2019·Journal of Cellular Biochemistry·Soudeh Ghafouri-FardVahid Kholghi Oskooei
Jan 30, 2021·Cell and Tissue Research·MacKinsey Johnson, Chishimba Nathan Mowa
Aug 4, 2021·Acta Biochimica Et Biophysica Sinica·Liyuan CuiMeirong Du

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Methods Mentioned

BETA
PCR
electrophoresis
FCS

Software Mentioned

Affymetrix
GeneChip
SpotFire
Excel

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