Microarray analysis reveals differential gene expression in hybrid sunflower species.

Molecular Ecology
Zhao LaiLoren H Rieseberg

Abstract

This paper describes the creation of a cDNA microarray for annual sunflowers and its use to elucidate patterns of gene expression in Helianthus annuus, Helianthus petiolaris, and the homoploid hybrid species Helianthus deserticola. The array comprises 3743 ESTs (expressed sequence tags) representing approximately 2897 unique genes. It has an average clone/EST identity rate of 91%, is applicable across species boundaries within the annual sunflowers, and shows patterns of gene expression that are highly reproducible according to real-time RT-PCR (reverse transcription-polymerase chain reaction) results. Overall, 12.8% of genes on the array showed statistically significant differential expression across the three species. Helianthus deserticola displayed transgressive, or extreme, expression for 58 genes, with roughly equal numbers exhibiting up- or down-regulation relative to both parental species. Transport-related proteins were strongly over-represented among the transgressively expressed genes, which makes functional sense given the extreme desert floor habitat of H. deserticola. The potential adaptive value of differential gene expression was evaluated for five genes in two populations of early generation (BC2) hybrids betwe...Continue Reading

References

Oct 15, 1982·JAMA : the Journal of the American Medical Association·E M RogersC U Lowe
Dec 3, 1999·Heredity·L H RiesebergR K Wayne
Mar 14, 2000·Current Opinion in Plant Biology·T Richmond, S Somerville
Jun 10, 2000·Heredity·C A BuerkleL H Rieseberg
Aug 11, 2000·Evolution; International Journal of Organic Evolution·L H Rieseberg
Sep 21, 2000·BioTechniques·P HegdeJ Quackenbush
Jan 5, 2001·Nucleic Acids Research·R G HalgrenT R Zacharewski
Apr 20, 2001·Nature·J Knight
Jun 1, 2001·Human Mutation·W Xiao, P J Oefner
Jun 14, 2001·Trends in Ecology & Evolution·L H. Rieseberg
Jul 11, 2001·Genome Génome / Conseil National De Recherches Canada·B LiuJ F Wendel
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·M A NoorJ Reiland
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·N C EllstrandL H Rieseberg
Feb 28, 2002·Plant Molecular Biology·Asaph Aharoni, Oscar Vorst
Mar 21, 2002·Molecular Ecology·Greg Gibson
Apr 16, 2002·Science·Wolfgang EnardSvante Pääbo
Jul 24, 2002·Genetics·John M BurkeLoren H Rieseberg
Sep 5, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Tanya M GottliebSuzanne L Rutherford
Sep 12, 2002·Annual Review of Plant Biology·Jian-Kang Zhu
Dec 3, 2002·Science·Duncan GreigMichael Travisano
Mar 5, 2003·Trends in Genetics : TIG·Thomas C OsbornRobert A Martienssen
Apr 1, 2003·Proceedings of the National Academy of Sciences of the United States of America·Keith L AdamsJonathan F Wendel
Aug 9, 2003·Science·Loren H RiesebergChristian Lexer
Oct 25, 2003·Evolution; International Journal of Organic Evolution·Christian LexerLoren H Rieseberg
May 29, 2004·Genetics·John M BurkeLoren H Rieseberg
Jun 23, 2004·The Plant Cell·Guillaume Blanc, Kenneth H Wolfe
Jul 9, 2004·Genome Biology·Christopher Lee, Meenakshi Roy
Jul 28, 2004·The American Naturalist·Briana L GrossLoren H Rieseberg
Dec 25, 2004·The Journal of Heredity·B L Gross, L H Rieseberg
Jan 7, 2005·The Plant Journal : for Cell and Molecular Biology·Andreas MadlungLuca Comai
Feb 9, 2005·Evolution; International Journal of Organic Evolution·Fulco LudwigLisa A Donovan
Feb 22, 2005·The New Phytologist·Matthew J Hegarty, Simon J Hiscock

❮ Previous
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Citations

Apr 19, 2011·Functional & Integrative Genomics·Annie Archambault, Martina V Strömvik
Aug 10, 2007·Heredity·C R LandryJ M Ranz
Nov 6, 2013·Proceedings of the National Academy of Sciences of the United States of America·Wei WeiYan Zhao
Jun 27, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Matthew J HegartySimon J Hiscock
Jan 24, 2007·Genetics·June B NasrallahMikhail E Nasrallah
Jun 25, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Sebastien RenautLoren H Rieseberg
Oct 24, 2014·BMC Genomics·Luis G León-NoveloRita M Graze
Mar 11, 2008·Current Opinion in Plant Biology·Sophie Karrenberg, Alex Widmer
Oct 16, 2007·Current Opinion in Genetics & Development·Eric J Baack, Loren H Rieseberg
Feb 14, 2007·Trends in Ecology & Evolution·Jan E KammengaRainer Breitling
Dec 26, 2006·Trends in Genetics : TIG·Zachary B Lippman, Dani Zamir
Jan 30, 2009·Molecular Ecology·Matthew J HegartySimon J Hiscock
Jan 22, 2008·Molecular Ecology·Loren Rieseberg, Harry Smith
Apr 22, 2006·Molecular Ecology·Carol Eunmi Lee, Thomas Mitchell-Olds
Dec 24, 2013·Ecology and Evolution·Kathryn A Stewart, Stephen C Lougheed
Apr 17, 2013·The New Phytologist·Alessia GuggisbergLoren H Rieseberg
Jun 8, 2010·Plant, Cell & Environment·Erin T HamanishiMalcolm M Campbell
Jun 2, 2012·Molecular Ecology·Till CzypionkaArne W Nolte
Sep 27, 2012·Molecular Ecology·M J Hegarty

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