Microarray-based analysis of cell-cycle gene expression during spermatogenesis in the mouse.

Biology of Reproduction
Dipanwita Roy ChoudhuryJohn R McCarrey

Abstract

Mammalian spermatogenesis is a continuum of cellular differentiation in a lineage that features three principal stages: 1) a mitotically active stage in spermatogonia, 2) a meiotic stage in spermatocytes, and 3) a postreplicative stage in spermatids. We used a microarray-based approach to identify changes in expression of cell-cycle genes that distinguish 1) mitotic type A spermatogonia from meiotic pachytene spermatocytes and 2) pachytene spermatocytes from postreplicative round spermatids. We detected expression of 550 genes related to cell-cycle function in one or more of these cell types. Although a majority of these genes were expressed during all three stages of spermatogenesis, we observed dramatic changes in levels of individual transcripts between mitotic spermatogonia and meiotic spermatocytes and between meiotic spermatocytes and postreplicative spermatids. Our results suggest that distinct cell-cycle gene regulatory networks or subnetworks are associated with each phase of the cell cycle in each spermatogenic cell type. In addition, we observed expression of different members of certain cell-cycle gene families in each of the three spermatogenic cell types investigated. Finally, we report expression of 221 cell-cycl...Continue Reading

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Citations

Sep 4, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Zhangsheng YangJohn R McCarrey
Jul 16, 2013·Asian Journal of Andrology·Tao ZhouXue-Jiang Guo
Apr 12, 2013·Human Reproduction Update·David MortimerCharles H Muller
Dec 3, 2014·Stem Cell Research·Daniel J CooperJohn R McCarrey
Apr 14, 2016·Development·Dawn L LovelaceBrian P Hermann
Jul 30, 2021·Gene Expression Patterns : GEP·Qixian LiXiaowei Guan

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Methods Mentioned

BETA
electrophoresis
PCR
ubiquitination
chip

Software Mentioned

GermOnline
ExPASy Analysis System
GeneSpring
Affymetrix
GeneSpring GX
NetAffx
RealTimeDesign
BLASTP

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