Microarray-based in vitro test system for the discrimination of contact allergens and irritants: identification of potential marker genes

Clinical Chemistry
S SzameitC Noehammer

Abstract

Animal tests have been used to characterize the potential of chemicals to produce allergic contact dermatitis, but this approach is increasingly a matter of public and political concern. Our aim was to develop and validate an alternative in vitro test that can identify contact allergens. We developed a targeted microarray containing oligonucleotide probes for 66 immune-relevant genes and analyzed gene expression in monocyte-derived dendritic cells (Mo-DCs) treated with 1 irritant (SDS) and 2 prominent contact allergens, nickel and Bandrowski's base (BB), which is the oxidation product of the most important hair dye allergen, p-phenylenediamine. Comparing RNA amounts in chemical-treated and solvent-treated cells, we identified significant changes in the expression of 21 genes and 10 genes after exposure of immature DCs (iDCs) to nickel and BB, respectively, but not after exposure to SDS. Eight genes were differentially expressed after application of both nickel and BB. Real-time PCR was used to confirm the results for selected genes. We propose a microarray-based in vitro test that might allow the identification of contact allergens. Independently from donor variability, several immune-relevant genes were up- or downregulated af...Continue Reading

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Citations

Sep 4, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Kazutoshi SaitoNaohiro Nishiyama
Jul 8, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S VerstraelenR Van Den Heuvel
Mar 24, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S SzameitC Noehammer
Nov 10, 2009·Expert Review of Molecular Diagnostics·Sandra SzameitChrista Noehammer
Jan 7, 2020·Alternatives to Laboratory Animals : ATLA·Bianca MariglianiAnna Maria Buehler
Nov 26, 2008·Alternatives to Laboratory Animals : ATLA·Erwin RoggenSilvia Casati

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