Microarray-based transcriptome of Listeria monocytogenes adapted to sublethal concentrations of acetic acid, lactic acid, and hydrochloric acid

Canadian Journal of Microbiology
Girum Tadesse TessemaLars Axelsson

Abstract

Listeria monocytogenes , an important foodborne pathogen, commonly encounters organic acids in food-related environments. The transcriptome of L. monocytogenes L502 was analyzed after adaptation to pH 5 in the presence of acetic acid, lactic acid, or hydrochloric acid (HCl) at 25 °C, representing a condition encountered in mildly acidic ready-to-eat food kept at room temperature. The acid-treated cells were compared with a reference culture with a pH of 6.7 at the time of RNA harvesting. The number of genes and magnitude of transcriptional responses were higher for the organic acids than for HCl. Protein coding genes described for low pH stress, energy transport and metabolism, virulence determinates, and acid tolerance response were commonly regulated in the 3 acid-stressed cultures. Interestingly, the transcriptional levels of histidine and cell wall biosynthetic operons were upregulated, indicating possible universal response against low pH stress in L. monocytogenes. The opuCABCD operon, coding proteins for compatible solutes transport, and the transcriptional regulator sigL were significantly induced in the organic acids, strongly suggesting key roles during organic acid stress. The present study revealed the complex trans...Continue Reading

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Citations

Apr 2, 2013·Canadian Journal of Microbiology·James L SmithGeorge C Paoli
Dec 10, 2014·International Journal of Molecular Sciences·Xuejiao SunLanming Chen
Mar 21, 2015·Food Microbiology·Laurel S BurallAtin R Datta
Nov 22, 2015·Applied and Environmental Microbiology·Samuel McCarthyPaul Blum
Jul 26, 2015·Applied and Environmental Microbiology·Silin TangTeresa M Bergholz

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