Microarray detection of gene overexpression in primary spontaneous pneumothorax

Experimental Lung Research
Hsin-Yuan FangWen-Je Ko

Abstract

Primary spontaneous pneumothorax (PSP) often occurs after the rupture of small bullae or subpleural blebs in otherwise normal lungs. The underlying mechanism(s) remain unclear. The aim of this study was to identify genes potentially involved in the development of PSP. Microarray analysis was performed to identify specific gene expression patterns. Expression levels of genes identified to be significantly up- or down-regulated in association with PSP were confirmed by real-time polymerase chain reaction (qRT-PCR) and Western blotting. Immunohistochemistry was performed to identify lung cell types highly expressing these genes. Microarray analysis revealed that expression levels of hypoxia-inducible factor-3 alpha (HIF-3alpha) and caspase-8 were significantly up-regulated in tissue from patients with PSP, whereas interferon-gamma, interleukin (IL)-6, and IL-8 were down-regulated (all P < .05). These genes are related to hypoxia, apoptosis, and inflammation. HIF-3alpha and caspase-8 protein levels were increased in samples from patients with PSP. HIF-3alpha and caspase-8 were localized in mesothelial cells, alveolar type II pneumocytes, and bronchoalveolar epithelial cells in samples from patients with PSP. Our findings, although ...Continue Reading

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Mar 26, 2013·American Journal of Respiratory Cell and Molecular Biology·Tianju LiuSem H Phan
Feb 20, 2014·Respiratory Physiology & Neurobiology·Alessandra S N T EliasCristiane S N B Garcia
Oct 5, 2011·Journal of Autism and Developmental Disorders·Dario SiniscalcoNicola Antonucci
Jul 13, 2014·Interactive Cardiovascular and Thoracic Surgery·Frank Cheau-Feng LinStella Chin-Shaw Tsai

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