Abstract
Primary spontaneous pneumothorax (PSP) often occurs after the rupture of small bullae or subpleural blebs in otherwise normal lungs. The underlying mechanism(s) remain unclear. The aim of this study was to identify genes potentially involved in the development of PSP. Microarray analysis was performed to identify specific gene expression patterns. Expression levels of genes identified to be significantly up- or down-regulated in association with PSP were confirmed by real-time polymerase chain reaction (qRT-PCR) and Western blotting. Immunohistochemistry was performed to identify lung cell types highly expressing these genes. Microarray analysis revealed that expression levels of hypoxia-inducible factor-3 alpha (HIF-3alpha) and caspase-8 were significantly up-regulated in tissue from patients with PSP, whereas interferon-gamma, interleukin (IL)-6, and IL-8 were down-regulated (all P < .05). These genes are related to hypoxia, apoptosis, and inflammation. HIF-3alpha and caspase-8 protein levels were increased in samples from patients with PSP. HIF-3alpha and caspase-8 were localized in mesothelial cells, alveolar type II pneumocytes, and bronchoalveolar epithelial cells in samples from patients with PSP. Our findings, although ...Continue Reading
References
Dec 1, 1996·Nature Medicine·S StrandP R Galle
Feb 7, 1997·Cell·S Nagata
Mar 23, 2000·The New England Journal of Medicine·S A Sahn, J E Heffner
May 16, 2000·Chest·D Weissberg, Y Refaely
Jul 19, 2000·Thorax·D GuptaD Strachan
Jan 6, 2001·The American Journal of Pathology·G Matute-BelloW C Liles
Sep 28, 2001·Biochemical and Biophysical Research Communications·S HaraN Imura
May 9, 2003·The Annals of Thoracic Surgery·Adel K Ayed
May 24, 2003·Trends in Molecular Medicine·Simone MocellinFrancesco M Marincola
Jun 14, 2003·Current Opinion in Pulmonary Medicine·Marc Noppen
Aug 29, 2003·Journal of Biochemistry·Yasuo KikuchiYoshiaki Fujii-Kuriyama
Sep 27, 2003·Respiration; International Review of Thoracic Diseases·Marc Noppen, Michael H Baumann
Jun 12, 2004·American Journal of Surgery·Takeshi MorimotoTsuguya Fukui
May 20, 2005·The Journal of Laboratory and Clinical Medicine·Nadia N HanselGregory B Diette
Oct 29, 2005·American Journal of Respiratory Cell and Molecular Biology·Jin-Tang ChenChih-Yi Chen
Feb 3, 2006·The Keio Journal of Medicine·Qing LuSharon Rounds
Feb 18, 2006·Molecular Cell·Liping LiuM Celeste Simon
Sep 2, 2006·The European Respiratory Journal·J-M TschoppP Astoul
Feb 3, 2007·Advances in Experimental Medicine and Biology·Maurizio Provenzano, Simone Mocellin
Citations
Mar 26, 2013·American Journal of Respiratory Cell and Molecular Biology·Tianju LiuSem H Phan
Jun 12, 2013·PloS One·Marta GabasaJavier Pereda
Feb 20, 2014·Respiratory Physiology & Neurobiology·Alessandra S N T EliasCristiane S N B Garcia
Oct 5, 2011·Journal of Autism and Developmental Disorders·Dario SiniscalcoNicola Antonucci
May 6, 2015·Molecular Medicine Reports·Sheng-Li YangXiefan Fang
Jul 13, 2014·Interactive Cardiovascular and Thoracic Surgery·Frank Cheau-Feng LinStella Chin-Shaw Tsai
Feb 8, 2011·European Journal of Pharmacology·Catia GiordanoVito de Novellis
Mar 8, 2013·PloS One·Ching-Yuang LinJeng-Shou Chang
Aug 15, 2020·Medicine·Chien-Kuang ChenHsin-Yuan Fang