Microarray glass slides coated with block copolymer brushes obtained by reversible addition chain-transfer polymerization

Analytical Chemistry
Giovanna PirriAlessandra Meo

Abstract

The reversible addition-fragmentation chain-transfer polymerization was used to prepare microarray slides grafted with polymer brushes for DNA-based applications. Block copolymer brushes of N,N-dimethylacrylamide (DMA) and glycidyl methacrylate (GMA), poly(DMA-b-GMA) were prepared by extending living poly(dimethylacrylamide) chains. The functional surface was used as a substrate for oligonucleotide hybridization experiments. The results were compared to those provided by glass slides coated by a self-assembled monolayer made of (3-glycidyloxypropyl)trimethoxysilane. Surfaces coated with block polymer brushes bearing oxirane groups are more efficient as substrates for oligonucleotide hybridization than surfaces coated with nonpolymeric self-assembled monolayers containing the same functional group. The high probe grafting density and hybridization efficiency achieved with this polymeric coating reveal the importance of the block architecture to ensure good accessibility of the immobilized probe. The new surface was characterized by static angle measurements and diffuse reflectance FT-IR spectroscopy on a silica model system.

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Citations

Mar 13, 2009·Analytical and Bioanalytical Chemistry·Julie M Goddard, David Erickson
Sep 23, 2010·Analytical and Bioanalytical Chemistry·Benedikt AsbachMichael Schäferling
Jul 20, 2010·Annual Review of Analytical Chemistry·Parul JainMerlin L Bruening
Mar 14, 2009·Colloids and Surfaces. B, Biointerfaces·Zheng-Chun LiuZhen-Cheng Chen
Dec 7, 2007·Colloids and Surfaces. B, Biointerfaces·Ryoko IwataKazunari Akiyoshi
Feb 12, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Marcella ChiariClaudio Oldani
Jul 17, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Yasuhiko IwasakiRyoko Iwata
Aug 29, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Yaming YuWolfgang Knoll
Sep 22, 2009·Chemical Reviews·Cyrille BoyerSébastien Perrier
Sep 24, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Nico ZammarelliRalf Weberskirch
Jan 15, 2014·ACS Applied Materials & Interfaces·Zhuang XieZijian Zheng
Jun 15, 2007·Analytical Chemistry·Chun-Hua LuXiao-Ru Wang

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