Microarray profiling of circular RNAs in steroid-associated osteonecrosis of the femoral head: Observational study.

Medicine
Tao YaoChao Cheng

Abstract

The aim of this study was to elucidate the molecular mechanisms and to identify the differential expression of circular RNAs (circRNAs) for steroid-associated osteonecrosis of the femoral head (SONFH) using bioinformatics analysis.circRNA microarray was performed with 3 SONFH tissues and the adjacent normal tissues, and differentially expressed circRNA were identified by limma package in R. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed using the Database for Annotation, Visualization and Integrated Discovery database. In addition, a differentially expressed genes (DEG)-associated circRNA/microRNA (miRNA) interaction was predicted by combination of TargetScan and miRanda, and the circRNA/miRNA interaction network generated by the cytoscape software.A total of 647 differentially expressed circRNAs, including 433 upregulated and 214 downregulated circRNA were identified. The most enriched GO terms for upregulated and downregulated circRNA were extracellular matrix organization and leukocyte activation in biological process; extracellular matrix and spindle pole in cellular component; integrin binding and ATP binding in molecular function, and KEGG pathway enrichmen...Continue Reading

References

May 12, 2009·The Journal of Steroid Biochemistry and Molecular Biology·Mohammad Amin KerachianEdward J Harvey
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Methods Mentioned

BETA
total hip arthroplasty
Feature Extraction
phosphotransferase

Software Mentioned

Feature Extraction
Volcano Plot
R
cytoscape
miRanda
Ingenuity Pathways Analysis
TargetScan

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