Microarray profiling of human white adipose tissue after exogenous leptin injection

European Journal of Clinical Investigation
S TalebW H M Saris

Abstract

Leptin is a secreted adipocyte hormone that plays a key role in the regulation of body weight homeostasis. The leptin effect on human white adipose tissue (WAT) is still debated. The aim of this study was to assess whether the administration of polyethylene glycol-leptin (PEG-OB) in a single supraphysiological dose has transcriptional effects on genes of WAT and to identify its target genes and functional pathways in WAT. Blood samples and WAT biopsies were obtained from 10 healthy nonobese men before treatment and 72 h after the PEG-OB injection, leading to an approximate 809-fold increase in circulating leptin. The WAT gene expression profile before and after the PEG-OB injection was compared using pangenomic microarrays. Functional gene annotations based on the gene ontology of the PEG-OB regulated genes were performed using both an 'in house' automated procedure and GenMAPP (Gene Microarray Pathway Profiler), designed for viewing and analyzing gene expression data in the context of biological pathways. Statistical analysis of microarray data revealed that PEG-OB had a major down-regulated effect on WAT gene expression, as we obtained 1,822 and 100 down- and up-regulated genes, respectively. Microarray data were validated us...Continue Reading

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Citations

Oct 14, 2008·Genes & Nutrition·L MaciaI Wolowczuk
Jul 3, 2013·BMC Bioinformatics·Irina DinuSaumyadipta Pyne
Jul 11, 2009·Fertility and Sterility·Henry BohlerStephen J Winters
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Sep 1, 2015·Adipocyte·Gary J HausmanLaura Lee-Rutherford
May 3, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kent LundholmIngvar Bosaeus
Oct 18, 2007·Current Opinion in Endocrinology, Diabetes, and Obesity

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