Microarrays of synthetic heparin oligosaccharides

Journal of the American Chemical Society
Jose L de PazPeter H Seeberger

Abstract

We present the first preparation of microarrays containing synthetic heparin oligosaccharides in order to elucidate the heparin-protein interactions involved in a variety of biological processes. For this purpose, we have developed a novel linker strategy that is compatible with the protecting-group manipulations required for the synthesis of the highly sulfated oligosaccharides and can also be extended to an automated solid phase approach. Strategic placement of the orthogonally protected amine linker was key to the success of the array construction. These heparin chips allow for the high-throughput screening of oligosaccharides by using approximately picomoles of protein. The potential of the new method was demonstrated by probing the carbohydrate affinity of two heparin-binding growth factors, FGF-1 and FGF-2, that are implicated in the development and differentiation of several tumors.

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Citations

Nov 30, 2006·Applied Microbiology and Biotechnology·Jian Liu, Lars C Pedersen
Nov 4, 2011·Applied Microbiology and Biotechnology·Ujjwal BhaskarRobert J Linhardt
Oct 12, 2010·Bioconjugate Chemistry·Avijit Kumar AdakAlexander Wei
Mar 22, 2012·Bioconjugate Chemistry·Francisco Javier Lopez-JaramilloFrancisco Santoyo-Gonzalez
Nov 23, 2007·ACS Chemical Biology·Jose L de PazPeter H Seeberger
Oct 5, 2007·Journal of the American Chemical Society·Tülay Polat, Chi-Huey Wong
Dec 18, 2012·Organic Letters·Steen U HansenJohn M Gardiner
Aug 22, 2006·Nature Chemical Biology·Jeremy E Turnbull, Robert J Linhardt
Aug 1, 2006·Nature Chemical Biology·Cristal I GamaLinda C Hsieh-Wilson
Jul 21, 2006·Chemical Communications : Chem Comm·Jose L de PazPeter H Seeberger
Mar 7, 2007·Organic & Biomolecular Chemistry·Hai Yu, Xi Chen
Jun 24, 2008·Chemical Society Reviews·Tim Horlacher, Peter H Seeberger
May 22, 2008·Molecular BioSystems·Emiliano GemmaAlison N Hulme
Jun 20, 2008·Molecular BioSystems·Jose L de Paz, Peter H Seeberger
Sep 20, 2008·Chemical Communications : Chem Comm·Sungjin ParkInjae Shin
Sep 20, 2008·Chemical Communications : Chem Comm·Nicolas LaurentSabine L Flitsch
Sep 17, 2009·Molecular BioSystems·Richard D Cummings
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Mar 8, 2013·Carbohydrate Polymers·Anaïs A E ChavarocheGerrit Eggink

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