Microautoradiography of [123I]ADAM in mice treated with fluoxetine and serotonin reuptake inhibitors

Nuclear Medicine and Biology
Xin-Xian YeJeng-Jong Hwang

Abstract

A radiopharmaceutical, (123)I-labeled 2-((2-((dimethylamino)methyl)phenyl)thio)-5-iodophenylamine ([(123)I]ADAM), has been developed recently for evaluation of how serotonin transporters (SERT) function in the brain. However, the detailed biodistribution and specific binding in certain brain areas are not well investigated. In this study, both phosphor plate imaging and microautoradiography were applied to explore the binding characteristics of [(123)I]ADAM in SERT neurons. The effect of two psychotropics and one narcotic on the binding of [(123)I]ADAM to SERT was also studied. Fluoxetine and desipramine, both are psychotropics and specific SERT ligands and decreased the affinity of [(123)I]ADAM, while p-chloroamphetamine (PCA), a narcotic, destroyed most of serotonergic neurons, as well as reducing the concentration of serotonin and the number of SERT in the brain as shown by the biodistribution of [(123)I]ADAM. Significant and selective accumulation of [(123)I]ADAM in the areas from midbrain to brain stem in normal mice with maximum target-to-background ratio was found at 90 minutes postinjection. A rapid clearance of [(131)I]ADAM at 120 minutes postinjection was found in the CA1, CA3 and ThN brain areas. In addition, the inh...Continue Reading

Citations

Feb 20, 2009·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Kuo-Hsing MaWen-Sheng Huang
Apr 17, 2013·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·Chun-Kai FangJeng-Jong Hwang
Feb 26, 2009·Psychiatry Research·Yuan-Hwa ChouShyh-Jen Wang
Mar 21, 2006·Nuclear Medicine and Biology·Jan Booij, Maartje M L de Win

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