Microbial amino acid synthesis and utilization in rats: incorporation of 15N from 15NH4Cl into lysine in the tissues of germ-free and conventional rats

The British Journal of Nutrition
David TorrallardonaMalcolm F Fuller

Abstract

The absorption of lysine synthesised by the gastrointestinal microflora was estimated by comparing the 15N incorporated into body lysine in four germ-free (15N-GF) and four conventional (15N-CV) rats. They were fed for 10 d on a protein-free diet containing fermentable carbohydrates and 15NH4Cl; another four conventional rats (control), fed on the same diet but with unlabelled NH4Cl, were used to estimate the natural abundance of 15N. The eviscerated carcass of each rat was homogenized and a sample hydrolysed. Lysine was isolated by ion-exchange chromatography and its 15N enrichment was measured by isotoperatio mass spectrometry. The 15N-CV rats significantly incorporated 15N into their body lysine. The 15N-GF rats had a statistically significant, although small, incorporation of 15N into their body lysine, probably arising from a measurement artifact. It was concluded, therefore, that all [15N]lysine was of microbial origin. The total lysine content in the body and the 15N enrichment of lysine in the microbial fraction of the faeces of the 15N-CV rats were also determined. The amount of microbial lysine absorbed by the 15N-CV rats was estimated by dividing the total amount of [15N]lysine in the body by the enrichment of microb...Continue Reading

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Citations

Oct 12, 1999·The American Journal of Physiology·C C MetgesV R Young
Jul 8, 2003·The British Journal of Nutrition·Peter FaberGerald E Lobley
Nov 10, 2005·The British Journal of Nutrition·Cornelia C Metges, Klaus J Petzke
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Nov 1, 1996·The British Journal of Nutrition·D TorrallardonaM F Fuller
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Apr 4, 2003·The Journal of Nutrition·David TorrallardonaMalcolm F Fuller
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Jun 27, 2000·The Journal of Nutrition·C C Metges
Apr 3, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Michael A Singer

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