Microbial and Viral Communities and Their Antibiotic Resistance Genes Throughout a Hospital Wastewater Treatment System

Frontiers in Microbiology
Morgan PetrovichGeorge Wells

Abstract

Antibiotic resistance poses a serious threat to global public health, and antibiotic resistance determinants can enter natural aquatic systems through discharge of wastewater effluents. Hospital wastewater in particular is expected to contain high abundances of antibiotic resistance genes (ARGs) compared to municipal wastewater because it contains human enteric bacteria that may include antibiotic-resistant organisms originating from hospital patients, and can also have high concentrations of antibiotics and antimicrobials relative to municipal wastewater. Viruses also play an important role in wastewater treatment systems since they can influence the bacterial community composition through killing bacteria, facilitating transduction of genetic material between organisms, and modifying the chromosomal content of bacteria as prophages. However, little is known about the fate and connections between ARGs, viruses, and their associated bacteria in hospital wastewater systems. To address this knowledge gap, we characterized the composition and persistence of ARGs, dsDNA viruses, and bacteria from influent to effluent in a pilot-scale hospital wastewater treatment system in Israel using shotgun metagenomics. Results showed that ARGs...Continue Reading

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Citations

Sep 10, 2020·Folia Microbiologica·Martina BorčinováPavel Kyslík
Sep 5, 2020·Antibiotics·Maciej ŻaczekAndrzej Górski
Oct 21, 2020·Antibiotics·Florence Hammer-DedetPatricia Licznar-Fajardo
Jul 30, 2020·The Science of the Total Environment·Xing-Pan GuoYi Yang
Aug 3, 2021·Environmental Science & Technology·Suraj GuptaPeter Vikesland
Apr 18, 2020·Ecotoxicology and Environmental Safety·Mutshiene Deogratias EkwanzalaMaggy Ndombo Benteke Momba
Nov 12, 2021·The Science of the Total Environment·Haikun ZhangXiaoke Hu

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Datasets Mentioned

BETA
PRJNA526679

Methods Mentioned

BETA
X-ray

Software Mentioned

Blast
CutAdapt
FastQC
Scikit
Bio Python package
Bowtie2
Seaborn
Matplotlib
TK
IDBA

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