Microbial assay of N2 fixation rate, a simple alternate for acetylene reduction assay

MethodsX
Subhajit Das, Tarun Kumar De

Abstract

Nitrogen is an essential element for living creatures in every ecosystem but nitrogen cannot be absorbed by the plant itself directly from the atmosphere, so for nitrogen, plant depends on both free living and symbiotic microbes present in the soil. Nitrogen fixation potentiality of the soil thus reveals its fertility with respect to nitrogen. Researchers developed and modified techniques for measuring nitrogen fixation rate of the soil and acetylene reduction assay (ARA) technique became the most popular till now. At the same time this technique has few limitations especially for the researchers from third world country due to lack of special infrastructure in the laboratory and the most required instrument for this technique, gas chromatograph machine, is very expensive. Any alternation of this technique is deserved highly for the researchers from the developing countries. The present work/method explained a new approach for determination of nitrogen fixation rate and this new method was named as "Microbial bio-assay". In this technique nitrogen fixers were cultured in specific medium and condition and after required time of interval the amount of nitrogen fixed by them were calculated. Exploration of soil of the Sundarban ma...Continue Reading

Citations

Feb 26, 2021·Molecular Plant-microbe Interactions : MPMI·Dongying LiuGehong Wei
Jan 25, 2022·Molecular Plant-microbe Interactions : MPMI

❮ Previous
Next ❯

Methods Mentioned

BETA
acetylene
Assay

Software Mentioned

MINITAB

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.