Microbial Base Editing: A Powerful Emerging Technology for Microbial Genome Engineering.

Trends in Biotechnology
Yu WangMeng Wang

Abstract

Genome engineering is crucial for answering fundamental questions about, and exploring practical applications of, microorganisms. Various microbial genome-engineering tools, including CRISPR/Cas-enhanced homologous recombination (HR), have been developed, with ever-improving simplicity, efficiency, and applicability. Recently, a powerful emerging technology based on CRISPR/Cas-nucleobase deaminase fusions, known as base editing, opened new avenues for microbial genome engineering. Base editing enables nucleotide transition without inducing lethal double-stranded (ds)DNA cleavage, adding foreign donor DNA, or depending on inefficient HR. Here, we review ongoing efforts to develop and apply base editing to engineer industrially and clinically relevant microorganisms. We also summarize bioinformatics tools that would greatly facilitate guide (g)RNA design and sequencing data analysis and discuss the future challenges and prospects associated with this technology.

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Mar 29, 2021·Methods : a Companion to Methods in Enzymology·Li YangYanhui Yang
Jul 6, 2021·Frontiers in Microbiology·Longxiang WangDeqiang Duanmu

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