Microbial Butyrate Synthesis Indicates Therapeutic Efficacy of Azathioprine in IBD Patients.

Journal of Crohn's & Colitis
M EffenbergerH Tilg

Abstract

The microbial ecosystem seems to be an important player for therapeutic intervenption in inflammatory bowel disease [IBD]. We assessed longitudinal microbiome changes in IBD patients undergoing therapy with either azathioprine [AZA] or anti-tumour necrosis factor [anti-TNF] antibodies. We predicted the metabolic microbial community exchange and linked it to clinical outcome. Faecal and blood samples were collected from 65 IBD patients at baseline and after 12 and 30 weeks on therapy. Clinical remission was defined as Crohn's Disease Activity Index [CDAI] < 150 in Crohn´s disease [CD], partial Mayo score <2 in ulcerative colitis [UC], and faecal calprotectin values <150 µg/g and C-reactive protein <5 mg/dl. 16S rRNA amplicon sequencing was performed. To predict microbial community metabolic processes, we constructed multispecies genome-scale metabolic network models. Paired Bray-Curtis distance between baseline and follow-up time points was significantly different for UC patients treated with anti-TNF antibodies. Longitudinal changes in taxa composition at phylum level showed a significant decrease of Proteobacteria and an increase of Bacteroidetes in CD patients responding to both therapies. At family level, Lactobacilli were a...Continue Reading

References

Aug 9, 2002·The New England Journal of Medicine·Daniel K Podolsky
Feb 3, 2007·Journal of Proteome Research·Julian R MarchesiYulan Wang
Jul 16, 2008·Inflammatory Bowel Diseases·James D LewisJonas H Ellenberg
Oct 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Harry SokolPhilippe Langella
Mar 3, 2010·Annual Review of Immunology·Arthur KaserRichard S Blumberg
Mar 10, 2010·Nature Biotechnology·Jeffrey D OrthBernhard Ø Palsson
Jul 17, 2010·BMJ : British Medical Journal·Patrick F van RheenenVaclav Fidler
Apr 2, 2011·Journal of Crohn's & Colitis·Dirk EsserRobert J Spiegel
Feb 24, 2012·Clinical Pharmacology and Therapeutics·Ingrid OrdásWilliam J Sandborn
Aug 24, 2012·Lancet·Daniel C Baumgart, William J Sandborn
Sep 28, 2012·Genome Biology·Xochitl C MorganCurtis Huttenhower
Feb 25, 2014·Gastroenterology·Aleksandar D KosticDirk Gevers
Mar 19, 2014·Cell Host & Microbe·Dirk GeversRamnik J Xavier
Apr 5, 2014·PLoS Computational Biology·Paul J McMurdie, Susan Holmes
Sep 11, 2014·Digestive Diseases and Sciences·Ashwin N Ananthakrishnan
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Jan 20, 2015·Metabolomics : Official Journal of the Metabolomic Society·Jacob Tveiten BjerrumOle Haagen Nielsen
Sep 2, 2015·ILAR Journal·Christoph BeckerStefan Wirtz
May 24, 2016·Nature Methods·Benjamin J CallahanSusan P Holmes
Jul 28, 2016·Frontiers in Microbiology·Jessica D ForbesCharles N Bernstein
Oct 30, 2016·Gastroenterology·James D Lewis, Maria T Abreu
Nov 20, 2016·Gut·Pablo OliveraLaurent Peyrin-Biroulet
Nov 29, 2016·Nature Biotechnology·Stefanía MagnúsdóttirInes Thiele
Feb 14, 2017·Nature Microbiology·Jonas HalfvarsonJanet K Jansson
May 12, 2017·Cell Host & Microbe·Ashwin N AnanthakrishnanRamnik J Xavier
Aug 24, 2017·Nature Reviews. Gastroenterology & Hepatology·Heitor S P de SouzaDimitrios Iliopoulos
Jan 22, 2019·Nature Medicine·Bahtiyar YilmazAndrew J Macpherson
Jul 26, 2019·Nature Biotechnology·Evan BolyenJ Gregory Caporaso

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Citations

Jan 17, 2021·Nutrients·Charlotte M VerburgtJohan E Van Limbergen
Dec 18, 2020·Frontiers in Immunology·Tobias J DemetrowitschSilvio Waschina
Mar 7, 2021·Pathogens·Martina FranzinGabriele Stocco
Mar 12, 2021·Inflammatory Bowel Diseases·Vanessa Zaiatz BittencourtElizabeth J Ryan
Jul 28, 2021·Annual Review of Microbiology·Almut HeinkenInes Thiele

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