Microbial characterisation of polyhydroxyalkanoates storing populations selected under different operating conditions using a cell-sorting RT-PCR approach

Applied Microbiology and Biotechnology
Paulo C LemosValter Tandoi

Abstract

The identity of polyhydroxyalkanoates (PHA) storing bacteria selected under aerobic dynamic feeding conditions, using propionate as carbon source (reactor P), was determined by applying reverse transcriptase-polymerase chain reaction (RT-PCR) on micromanipulated cells and confirmed by fluorescence in situ hybridisation (FISH). Four genera, Amaricoccus, Azoarcus, Thauera and Paraccoccus were detected, the latter only rarely present. All the biomass was involved in PHA storage as shown by Nile Blue staining. By quantitative FISH, their specific amount was determined in this and two other systems using acetate as the carbon substrate (sequencing batch reactor [SBR] A and A1). SBR A and reactor P had the same sludge retention time (SRT, 10 days), while reactor A1 was operated with the SRT of 1 day and the double organic loading rate (OLR). Systems fed with acetate (41.1 +/- 2.2 and 49.4 +/- 1.4% total Bacteria, for A and A1, respectively) became enriched in Thauera independently on the SRT and OLR, while it was only present in a minor amount when propionate was used as a substrate (1.9 +/- 0.2% total Bacteria). Amaricoccus was present in both reactors operated at 10 days SRT, favoured in the one fed with propionate (61.4 +/- 1.9% t...Continue Reading

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Citations

Nov 13, 2008·Applied Microbiology and Biotechnology·Luisa S SerafimMaria A M Reis
Jan 7, 2014·Applied Biochemistry and Biotechnology·Sebnem OzdemirOzer Cinar
Dec 18, 2012·Environmental Technology·Orhan InceMustafa Kolukirik
Feb 22, 2014·Bioresource Technology·Diogo QueirósLuísa S Serafim
Jul 28, 2016·Critical Reviews in Environmental Science and Technology·Oskar ModinMalte Hermansson
Oct 3, 2015·Applied Microbiology and Biotechnology·Diogo QueirósLuísa S Serafim
Aug 11, 2015·New Biotechnology·Om Murugan JanarthananSteven Pratt
Apr 2, 2011·Biotechnology and Bioengineering·Yang JiangMark C M van Loosdrecht
Nov 12, 2010·Water Research·Yang JiangMark C M van Loosdrecht
Dec 17, 2016·Applied Biochemistry and Biotechnology·Bo WuMing-Xiong He
May 13, 2017·Journal of Industrial Microbiology & Biotechnology·Diogo QueirósPaulo C Lemos
Jan 17, 2012·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Asli S CigginDerin Orhon
Aug 10, 2021·The Science of the Total Environment·Mariana MatosMaria A M Reis

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