Microbial colonization of irradiated pathogenic yeast to catheter surfaces: Relationship between adherence, cell surface hydrophobicity, biofilm formation and antifungal susceptibility. A scanning electron microscope analysis

International Journal of Radiation Biology
Hala Abdallah FarragMona Mohamed Kamal

Abstract

Technological advances such as long-term indwelling catheters have created milieu in which infections are a major complication. Thus it is essential to be able to recognize, diagnose, and treat infections occurring in immunocompromised patients. Adherence assay and quantitation of biofilms was performed by a spectrophotometric method, hydrophobicity was evaluated by adhesion to p-xylene. The minimum inhibitory concentration (MIC) of Nystatin was carried out by a well dilution method. Out of 100 bladder cancer patients, 23 pathogenic yeast isolates were identified. The samples were taken from urinary catheters and urine collected from their attached drainage bags. Pathogenic yeast identified were species of Candida, Cryptococcus, Saccharomyces, Blastoschizomyces, Trichosporn, Hansenula, Prototheca and Rhodotorula. With the exception of Rhodotorula minuta, the yeast were sensitive to the antimycotic agent (Nystatin) used before and after in vitro gamma irradiation at 24.41 Gy as measured by a disc diffusion method. All tested yeast strains were slime producers and showed positive adherence reactions. There were considerable differences in adherence measurements after irradiation. An increase in adherence measurement values (using...Continue Reading

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Citations

Nov 8, 2017·Future Microbiology·Eliane M da SilvaTerezinha Ie Svidzinski
Dec 22, 2017·BMC Infectious Diseases·Eliane Martins da SilvaTerezinha Inez Estivalet Svidzinski

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