Microbial communities and fecal indicator bacteria associated with Cladophora mats on beach sites along Lake Michigan shores

Applied and Environmental Microbiology
Ola A OlapadeS L McLellan

Abstract

A high biomasses of Cladophora, a filamentous green alga, is found mainly during the summer along the shores of Lake Michigan. In this study, the abundance and persistence of the fecal indicator bacterium Escherichia coli and sulfate-reducing bacteria (SRB) on Cladophora mats collected at Lake Michigan beaches were evaluated using both culture-based and molecular analyses. Additionally, 16S rRNA gene cloning and sequencing were used to examine the bacterial community composition. Overall, E. coli was detected in all 63 samples obtained from 11 sites, and the average levels at most beaches ranged from 2,700 CFU/100 g (wet weight) of Cladophora to 7,500 CFU/100 g of Cladophora. However, three beaches were found to have site average E. coli densities of 12,800, 21,130, and 27,950 CFU/100 g of Cladophora. The E. coli levels in the lake water collected at the same time from these three sites were less than the recommended U.S. Environmental Protection Agency limit, 235 CFU/100 ml. E. coli also persisted on Cladophora mats in microcosms at room temperature for more than 7 days, and in some experiments it persisted for as long as 28 days. The SRB densities on Cladophora mats were relatively high, ranging from 4.4x10(6) cells/g (6.64 l...Continue Reading

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Citations

Mar 3, 2010·Environmental Monitoring and Assessment·Ana KovačićToni Zitko
Feb 19, 2013·Environmental Monitoring and Assessment·Ayokunle Christopher DadaRahimi Hamid
Nov 7, 2012·Folia Microbiologica·Lenka BrablcováMartin Rulík
Feb 21, 2013·Environmental Science & Technology·Chan Lan ChunMichael J Sadowsky
Jul 6, 2006·Applied and Environmental Microbiology·Satoshi IshiiMichael J Sadowsky
Oct 7, 2008·Applied and Environmental Microbiology·Elaine MoriartyBrent Gilpin
Apr 17, 2012·Journal of Environmental and Public Health·Robert W DerletCharles R Goldman
Jan 1, 2010·Microbes and Environments·Ola A Olapade, Elizabeth A Weage
Nov 11, 2014·Re/views in Environmental Science and Bio/technology·Richard WhitmanHelena M Solo-Gabriele
Sep 10, 2014·Trends in Microbiology·Sandra L McLellan, A Murat Eren
Dec 9, 2008·Water Research·Muruleedhara N ByappanahalliMichael J Sadowsky
Jul 24, 2007·Water Research·Katharine G Field, Mansour Samadpour
Apr 25, 2007·Water Research·Muruleedhara N ByappanahalliMichael J Sadowsky
Apr 29, 2009·Environmental Microbiology·Sarah P Walters, Katharine G Field
Feb 1, 2013·Journal of Phycology·Shahrizim B ZulkiflyLinda E Graham
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Sep 26, 2016·The Science of the Total Environment·Chan Lan ChunMichael J Sadowsky
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