Microbial communities in the garbage composting with rice hull as an amendment revealed by culture-dependent and -independent approaches

Journal of Bioscience and Bioengineering
Hiroaki TakakuMasamichi Takagi

Abstract

The diversity and succession of microbial communities during the garbage composting with rice hull as an amendment were studied by denaturing gradient gel electrophoresis (DGGE) and clone library analysis of PCR-amplified 16S ribosomal DNA (rDNA) with universal primers. Based on temperature changes, the composting process could be divided into thermophilic, cooling-down, and maturing stages. The DGGE profiles and clone library analysis revealed that the microbial community drastically changed during the composting process from the thermophilic to the maturing stages. The dominant bacterial group changed from the phylum Firmicutes in the thermophilic stage to the phylum Bacteroidetes in the maturing stage. This change in microbial communities may be significant for the composting process. The diversity of cultivated bacteria isolated from samples taken at various stages of the composting process was low. A total of 87 isolates were classified as belonging to only four different groups. These groups were also detected in the DGGE profiles and by the clone library analysis. Our study indicated that a combination of culture-dependent and -independent approaches could be very useful for monitoring both bacterial diversity and the su...Continue Reading

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Citations

Mar 31, 2010·BMC Microbiology·Pasi PartanenMartin Romantschuk
Sep 19, 2007·Microbial Ecology·Ivone Vaz-MoreiraOlga C Nunes
Sep 3, 2008·Microbial Ecology·Shah Md Asraful IslamHan Dae Yun
Dec 8, 2009·Applied Microbiology and Biotechnology·Balázs VajnaKároly Márialigeti
Jan 15, 2013·Journal of Applied Microbiology·H MiyamotoH Kodama
Jul 16, 2009·Journal of Applied Microbiology·O Le GoffN Wéry
Feb 28, 2016·Applied Microbiology and Biotechnology·Matthias Kästner, Anja Miltner
May 27, 2014·International Journal of Systematic and Evolutionary Microbiology·Sung Wook HongKun Sub Chung
Aug 13, 2011·Bioresource Technology·Weidong WangRuiyong Jing
Mar 12, 2013·Bioresource Technology·Vidya de GannesWilliam J Hickey
Oct 26, 2016·Journal of Bioscience and Bioengineering·Yukihiro TashiroKenji Sakai
Oct 19, 2016·Biotechnology for Biofuels·Martinus J A DaasRichard van Kranenburg
May 3, 2014·FEMS Microbiology Ecology·Ines VerbaendertPaul De Vos
Apr 3, 2008·Macromolecular Bioscience·Parveen Sangwan, Dong Yang Wu

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