Microbial Communities on Seafloor Basalts at Dorado Outcrop Reflect Level of Alteration and Highlight Global Lithic Clades

Frontiers in Microbiology
Michael D LeeBeth Orcutt

Abstract

Areas of exposed basalt along mid-ocean ridges and at seafloor outcrops serve as conduits of fluid flux into and out of a subsurface ocean, and microbe-mineral interactions can influence alteration reactions at the rock-water interface. Located on the eastern flank of the East Pacific Rise, Dorado Outcrop is a site of low-temperature (<20°C) hydrothermal venting and represents a new end-member in the current survey of seafloor basalt biomes. Consistent with prior studies, a survey of 16S rRNA gene sequence diversity using universal primers targeting the V4 hypervariable region revealed much greater richness and diversity on the seafloor rocks than in surrounding seawater. Overall, Gamma-, Alpha-, and Deltaproteobacteria, and Thaumarchaeota dominated the sequenced communities, together making up over half of the observed diversity, though bacterial sequences were more abundant than archaeal in all samples. The most abundant bacterial reads were closely related to the obligate chemolithoautotrophic, sulfur-oxidizing Thioprofundum lithotrophicum, suggesting carbon and sulfur cycling as dominant metabolic pathways in this system. Representatives of Thaumarchaeota were detected in relatively high abundance on the basalts in comparis...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Dec 3, 2003·Environmental Microbiology·Diana E NorthupClifford N Dahm
Feb 27, 2004·Nucleic Acids Research·Wolfgang LudwigKarl-Heinz Schleifer
Aug 2, 2005·Trends in Microbiology·Katrina J EdwardsThomas M McCollom
Sep 7, 2007·Environmental Microbiology·Olivia U MasonStephen J Giovannoni
Feb 16, 2008·Nature Reviews. Microbiology·Céline Brochier-ArmanetPatrick Forterre
May 30, 2008·Nature·Cara M SantelliKatrina J Edwards
Aug 12, 2008·Systematic and Applied Microbiology·Pablo YarzaRamon Rosselló-Móra
Sep 12, 2008·Environmental Microbiology·Cara M SantelliKatrina J Edwards
Mar 31, 2009·International Journal of Systematic and Evolutionary Microbiology·Dong Han ChoiByung Cheol Cho
Nov 1, 2004·FEMS Microbiology Ecology·Kristine LysnesRolf B Pedersen
Mar 9, 2010·Journal of Bacteriology·Kelly P WilliamsAllan W Dickerman
Apr 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·C B WalkerD A Stahl
Sep 28, 2010·Applied and Environmental Microbiology·Byoung-Joon ParkSung-Keun Rhee
Jun 28, 2011·Bioinformatics·Robert C EdgarRob Knight
Nov 19, 2011·Molecular Ecology·Lucie ZingerThomas Pommier
Nov 22, 2011·Applied and Environmental Microbiology·Erik S WrightDaniel R Noguera
May 5, 2012·Bioinformatics·Elmar PruesseFrank Oliver Glöckner
Aug 21, 2012·International Journal of Systematic and Evolutionary Microbiology·Mohammad Ali AmoozegarAntonio Ventosa
Dec 4, 2012·Nature Methods·Nicholas A BokulichJ Gregory Caporaso
Apr 2, 2013·Frontiers in Microbiology·Jason B SylvanKatrina J Edwards
Apr 20, 2014·International Journal of Systematic and Evolutionary Microbiology·Naoto TanakaValery V Mikhailov
Oct 7, 2015·Frontiers in Microbiology·Beth N OrcuttPeter R Girguis
Oct 1, 2001·Oecologia·Pierre Legendre, Eugene D Gallagher

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Citations

Jun 16, 2016·Frontiers in Microbiology·Steffen L Jørgensen, Rui Zhao
Nov 12, 2019·Environmental Microbiology·Katarina DamjanovicMadeleine J H van Oppen
May 21, 2020·Biostatistics·Amy D Willis, Bryan D Martin
Mar 12, 2020·MSystems·Rui ZhaoSteffen L Jørgensen
Sep 10, 2020·Frontiers in Microbiology·Tor Einar MøllerSteffen L Jørgensen
Oct 27, 2017·Extremophiles : Life Under Extreme Conditions·Christos A ChristakisAntonios Magoulas
Sep 3, 2019·Frontiers in Microbiology·Hannah E EpsteinMadeleine J H van Oppen
Aug 22, 2017·Frontiers in Microbiology·Jessica M LabontéBeth N Orcutt
Jun 29, 2018·Frontiers in Microbiology·Laura A ZinkeJan P Amend
Dec 22, 2020·Environmental Microbiology·Beth N OrcuttElizabeth Trembath-Reichert
Oct 19, 2021·Frontiers in Microbiology·Pauline BergstenViggó Thor Marteinsson

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Datasets Mentioned

BETA
SRP063681
FJ800194

Methods Mentioned

BETA
chip
Assay
PCR

Software Mentioned

Silva SINA
SILVA
Phylogenetic Placer (
RAxML
RStudio
vegan
Decipher
adonis
anosim
BLAST

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